1. Instructor: Colleen Malone Office Hours: After school by appointment only. E-mail Address: cmalone@conejo.k12.ca.us (email is the best way to contact me) Telephone: 498-3676 ext. 1307 Website: http://newburyparkhighschool.net/malone/

2. My Website

3. Who is Mrs. Malone? Careers: Texas A&M Health Science Center UCLA Amgen Newbury Park High School

4. Who is Mrs. Malone? Classes: Biology-CP Biology-H Marine Science-CP Biotechnology-CP

5. Warm Up (to review or introduce new material) Introduction/Demonstration/Current Events Lecture and Classroom Discussion Laboratory Work Audio Visual Connection and Summary

6. Introduction to biotechnology Biology review -(biochemistry, cells, DNA structure/replication; protein structure/synthesis) Chemistry review -molarity, making solutions, concentrations Techniques: Micropipeting, Gel electrophoresis (agarose gels), Growing bacteria (LB agar plates/ LB broth)

7. Gel Electrophoresis

8. Bacterial Cell Cultures

9. Amgen-Bruce Wallace Program OR Coastal Marine Biolabs (DNA Barcoding Project) Techniques: DNA extraction, Bacterial transformation, Polymerase Chain Reaction, DNA sequencing

10. DNA Extraction/Purification

11. Bacterial Transformation Special genes from fireflies, for instance, have been inserted into cells using the crown gall bacterium to produce glowing plants fireflies

12. PCR

13. DNA Sequencing

14. Grading will be based on the following percentages & using category weights. Classwork/Homework LATE Passes? 10% Tests/Quizzes55% Labs / Projects (includes lab notebook) 25% Final Exam/Project10%

15. Course grades will be determined by percent weights in several categories. The final grade will be based on the following percentages: 93 - 100A70-72C- 90-92A-63-69D 83 - 89B60-62D- 80-82B-0-59F 73-79C

16. Homework will be assigned on a regular basis; Monday through Friday. Even if a homework assignment has not been given, time should be spent each night reviewing what we did in class that day. Students are responsible for following the formal procedure for make-up work after an absence.

17. Reading scientific papers Writing scientific papers Organization Meeting deadlines Following directions Critical thinking Presenting data in public

18. Take an interest Have students do homework before social time / computer time Encourage study sessions Check day planner for important dates Check day planner for important dates Check grades on my website (Zangle) Check grades on my website (Zangle) http://newburyparkhighschool.net/malone/ E-mail me with questions and/or concerns