1. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings PowerPoint Lectures for Biology, Seventh Edition Neil Campbell and Jane Reece Lectures by Chris Romero Chapter 46 Animal Reproduction

2. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Overview: Doubling Up for Sexual Reproduction The two earthworms in this picture are mating Each worm produces sperm and eggs; in a few weeks, new worms will hatch from fertilized eggs Aspects of animal form and function can be viewed broadly as adaptations contributing to reproductive success

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4. Concept 46.1: Both asexual and sexual reproduction occur in the animal kingdom Asexual reproduction is creation of offspring whose genes all come from one parent Sexual reproduction is creation of offspring by fusion of male gametes (sperm) and female gametes (eggs) to form zygotes

5. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Mechanisms of Asexual Reproduction Many invertebrates reproduce asexually by fission, separation of a parent into two or more individuals of about the same size Video: Hydra Budding Video: Hydra Budding

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7. In budding, two new individuals arise from outgrowths of existing ones Fragmentation is breaking of the body into pieces, some or all of which develop into adults Fragmentation must be accompanied by regeneration, regrowth of lost body parts

8. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Reproductive Cycles and Patterns Most animals exhibit reproductive cycles related to changing seasons Reproductive cycles are controlled by hormones and environmental cues Animals may reproduce asexually or sexually, or they may alternate these methods

9. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Parthenogenesis is a process in which an egg develops without being fertilized Several genera of fishes, amphibians, and lizards reproduce only by a complex form of parthenogenesis

10. LE 46-3 Estrogen Ovulation Female- like Progesterone Ovary size Time Hormone level Ovulation Behavior Male- like Female- like Male- like

11. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Sexual reproduction is a special problem for organisms that seldom encounter a mate One solution is hermaphroditism, in which each individual has male and female reproductive systems In sequential hermaphroditism, an individual reverses its sex during its lifetime

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13. Concept 46.2: Fertilization depends on mechanisms that help sperm meet eggs of the same species The mechanisms of fertilization, the union of egg and sperm, play an important part in sexual reproduction In external fertilization, eggs shed by the female are fertilized by sperm in the external environment Video: Hydra Releasing Sperm Video: Hydra Releasing Sperm

14. LE 46-5 Eggs

15. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings In internal fertilization, sperm are deposited in or near the female reproductive tract, and fertilization occurs within the tract Fertilization requires critical timing, often mediated by environmental cues, pheromones, and/or courtship behavior Internal fertilization requires behavioral interactions and compatible copulatory organs

16. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Ensuring the Survival of Offspring All species produce more offspring than the environment can handle, but the proportion that survives is quite small The embryos of many terrestrial animals develop in eggs that can withstand harsh environments Many animals retain the embryo, which develops inside the female In many animals, parental care helps ensure survival of offspring

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18. Gamete Production and Delivery To reproduce sexually, animals must have systems that produce gametes The least complex systems do not contain distinct gonads, organs that produce gametes The most complex systems contain many sets of accessory tubes and glands that carry, nourish, and protect gametes and developing embryos Many animals with relatively simple body plans have highly complex reproductive systems

19. LE 46-7 Genital pore Male organs: Seminal vesicle Sperm duct (vas deferens) Vas efferens Testis (Excretory pore) (Digestive tract) Female organs: Uterus Yolk gland Yolk duct Oviduct Ovary Seminal receptacle

20. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Most insects have separate sexes with complex reproductive systems

21. LE 46-8 Male honeybee (drone) Seminal vesicle Vas deferens Testis Accessory gland Ejaculatory duct Penis Female honeybee (queen) Accessory gland Ovary Oviduct Vagina Spermatheca

22. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Concept 46.3: Reproductive organs produce and transport gametes: focus on humans

23. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Female Reproductive Anatomy The female external reproductive structures include the clitoris and two sets of labia The internal organs are a pair of gonads and a system of ducts and chambers that carry gametes and house the embryo and fetus Animation: Female Reproductive Anatomy Animation: Female Reproductive Anatomy

24. LE 46-9a Bartholin’s gland Vaginal opening Vagina Cervix (Rectum) Oviduct Ovary Uterus (Urinary bladder) (Pubic bone) Urethra Shaft Glans Prepuce Labia majora Clitoris Labia minora

25. LE 46-9b Vagina Cervix Oviduct Ovaries Uterus Endometrium Follicles Corpus luteum Uterine wall

26. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Ovaries The female gonads, the ovaries, lie in the abdominal cavity Each ovary is enclosed in a tough protective capsule and contains many follicles A follicle consists of one egg surrounded by one or more layers of follicle cells

27. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Ovulation expels an egg cell from the follicle The remaining follicular tissue grows within the ovary, forming a mass called the corpus luteum The corpus luteum secretes hormones, depending on whether pregnancy occurs

28. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Oviducts and Uterus The egg cell is released into the abdominal cavity near the opening of the oviduct, or fallopian tube Cilia in the tube convey the egg to the uterus

29. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Vagina and Vulva The vagina is a thin-walled chamber that is the repository for sperm during copulation and serves as the birth canal The vagina opens to the outside at the vulva, which includes the hymen, vestibule, labia minora, labia majora, and clitoris

30. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Mammary Glands The mammary glands are not part of the reproductive system but are important to mammalian reproduction Within the glands, small sacs of epithelial tissue secrete milk

31. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Male Reproductive Anatomy In most mammals, the male’s external reproductive organs are the scrotum and penis Internal organs are the gonads, which produce sperm and hormones, and accessory glands Animation: Male Reproductive Anatomy Animation: Male Reproductive Anatomy

32. LE 46-10b Seminal vesicle Urethra Scrotum Glans penis Testis Prostate gland Bulbourethral gland Erectile tissue of penis Epididymis (Urinary bladder) (Public bone) Prepuce Vas deferens Ejaculatory duct (Rectum)

33. LE 46-10a Seminal vesicle (behind bladder) Urethra Scrotum Glans penis Testis Prostate gland Bulbourethral gland Erectile tissue of penis Vas deferens Epididymis (Urinary bladder)

34. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Testes The male gonads, or testes, consist of highly coiled tubes surrounded by connective tissue Sperm form in these seminiferous tubules Production of normal sperm cannot occur at the body temperatures of most mammals The testes of many mammals are held outside the abdominal cavity in the scrotum, where the temperature is lower than in the abdominal cavity

35. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Ducts From the seminiferous tubules of a testis, sperm pass into the coiled tubules of the epididymis During ejaculation, sperm are propelled through the muscular vas deferens and the ejaculatory duct, and then exit the penis through the urethra

36. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Glands Three sets of accessory glands add secretions to the semen, the fluid that is ejaculated The two seminal vesicles contribute about 60% of the total volume of semen

37. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The prostate gland secretes its products directly into the urethra through several small ducts The bulbourethral gland secretes a clear mucus before ejaculation that neutralizes acidic urine remaining in the urethra

38. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Semen in the Female Reproductive Tract Within the female reproductive tract, a number of processes, including contractions of the uterus, help move the sperm up the uterus

39. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Penis The human penis is composed of three cylinders of spongy erectile tissue During sexual arousal, the erectile tissue fills with blood from the arteries, causing an erection

40. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Human Sexual Response Two reactions predominate in both sexes: – Vasocongestion, the filling of tissue with blood – Myotonia, increased muscle tension The sexual response cycle has four phases: excitement, plateau, orgasm, and resolution

41. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Concept 46.4: In humans and other mammals, a complex interplay of hormones regulates gametogenesis The process of gametogenesis is based on meiosis, but differs in females and males Oogenesis is development of mature ova Spermatogenesis is production of mature sperm

42. LE 46-11 Primary germ cell in embryo Mitotic division Primary oocyte Ovary Oogonium Differentiation Oogonium in ovary Primary oocyte within follicle Growing follicle Secondary oocyte Completion of meiosis I and onset of meiosis II Ovulation First polar body Mature follicle Entry of sperm triggers completion of meiosis II Second polar body Ovum Ruptured follicle Ovulated secondary oocyte Corpus luteum Degen- erating corpus luteum

43. LE 46-12 Spermatogonium Primary spermatocyte (in prophase of meiosis I) Mitotic division, producing large numbers of spermatogonia Meiosis I completed Differentiation and onset of meiosis I Secondary spermatocyte Meiosis II Early spermatids Spermatids (at two stages of differentiation) Differentiation Sperm cells Neck Head Midpiece Tail Plasma membrane Mitochondria AcrosomeNucleus Sertoli cell nucleus Lumen of Seminiferous tubule Cross section of seminiferous tubule Testis Epididymis Seminiferous tubule

44. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Oogenesis differs from spermatogenesis in three major ways: – During meiotic divisions, cytokinesis is unequal, with almost all of the cytoplasm in one daughter cell, the secondary oocyte – Unlike sperm, eggs are not produced continuously throughout life – Oogenesis has long “resting” periods, whereas sperm are produced in uninterrupted sequence

45. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The Reproductive Cycles of Females In females, the secretion of hormones and the reproductive events they regulate are cyclic

46. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Menstrual Versus Estrous Cycles Two different types of cycles occur in females Humans and other primates have menstrual cycles; other mammals have estrous cycles In both cases, ovulation occurs after the endometrium has started to thicken in preparation for implantation

47. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings In menstrual cycles: – The endometrium is shed from the uterus in a bleeding called menstruation – Sexual receptivity is not limited to a timeframe In estrous cycles: – The endometrium is reabsorbed by the uterus – Sexual receptivity is limited to a “heat” period

48. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The Human Female Reproductive Cycle: A Closer Look The female reproductive cycle is one integrated cycle involving two organs: the uterus and ovaries Secretion of GnRH from the hypothalamus and of FSH and LH from the anterior pituitary orchestrates the female reproductive cycle Five kinds of hormones participate in an elaborate scheme involving positive and negative feedback Animation: Post Ovulation Animation: Post Ovulation Animation: Ovulation Animation: Ovulation

49. LE 46-13 Hypothalamus Anterior pituitary Control by hypothalamus Pituitary gonadotropins in blood FSHLH FSH LH GnRH Inhibited by combination of estrogen and progesterone Inhibited by low levels of estrogen Stimulated by high levels of estrogen LH surge triggers ovulation FSH and LH stimulate follicle to grow Mature follicle Growing follicle Ovarian cycle Corpus luteum Degenerating corpus luteum Follicular phase OvulationLuteal phase Progesterone and estrogen secreted by corpus luteum Estrogen secreted by growing follicle in increasing amounts Progesterone and estro- gen promote thickening of endometrium Peak causes LH surge Ovarian hormones in blood Estrogen level very low Progesterone Estrogen Endometrium Uterine (menstrual) cycle Menstrual flow phaseProliferative phaseSecretory phase Days 28 2520 1514 105 0

50. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The Ovarian Cycle In the ovarian cycle, hormones stimulate follicle growth, which results in ovulation Following ovulation, the follicular tissue left behind transforms into the corpus luteum

51. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The Uterine (Menstrual) Cycle Cycle after cycle, the maturation and release of egg cells from the ovary are integrated with changes in the uterus If an embryo has not implanted in the endometrium by the end of the secretory phase, a new menstrual flow commences

52. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Menopause After about 450 cycles, human females undergo menopause, the cessation of ovulation and menstruation

53. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Hormonal Control of the Male Reproductive System Testosterone and other androgens are directly responsible for the primary and secondary sex characteristics of the male Androgen secretion and sperm production are controlled by hypothalamic and pituitary hormones Animation: Male Hormones Animation: Male Hormones

54. LE 46-14 Stimuli from other areas in the brain Anterior pituitary Hypothalamus Negative feedback Primary and secondary sex characteristics Leydig cells make testosterone LH Testis Sertoli cells Spermatogenesis GnRH FSH

55. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Concept 46.5: In humans and other placental mammals, an embryo grows into a newborn in the mother’s uterus Pregnancy, or gestation, is the condition of carrying one or more embryos in the uterus Duration of pregnancy in other species correlates with body size and maturity of the young at birth

56. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Conception, Embryonic Development, and Birth Conception, fertilization of an egg by a sperm, occurs in the oviduct After fertilization, the zygote undergoes cleavage and develops into a blastocyst before implantation in the endometrium

57. LE 46-15a From ovulation to implantation Cleavage starts Fertilization occurs Uterus Ovulation Ovary Endometrium The blastocyst implants Cleavage continues

58. LE 46-15b Implantation of blastocyst Blastocyst Endo- metrium Cavity Inner cell mass Trophoblast

59. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings First Trimester Human gestation can be divided into three trimesters of about three months each The first trimester is the time of most radical change for both the mother and the embryo

60. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings During its first 2 to 4 weeks, the embryo obtains nutrients directly from the endometrium Meanwhile, the outer layer of the blastocyst mingles with the endometrium and eventually forms the placenta Blood from the embryo travels to the placenta through arteries of the umbilical cord and returns via the umbilical vein

61. LE 46-16 Umbilical cord Chorionic villus containing fetal capillaries Maternal blood pools Uterus Placenta Maternal arteries Maternal veins Maternal portion of placenta Fetal portion of placenta (chorion) Umbilical arteries Umbilical vein Umbilical cord Fetal arteriole Fetal venule

62. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The first trimester is the main period of organogenesis, development of the body organs

63. LE 46-17 5 weeks.14 weeks. 20 weeks.

64. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Second Trimester During the second trimester – The fetus grows and is very active – The mother may feel fetal movements – The uterus grows enough for the pregnancy to become obvious

65. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Third Trimester During the third trimester, the fetus grows and fills the space within the embryonic membranes A complex interplay of local regulators and hormones induces and regulates labor, the process by which childbirth occurs

66. LE 46-18 Estrogen Oxytocin from ovaries from fetus and mother’s posterior pituitary Induces oxytocin receptors on uterus Stimulates uterus to contract Stimulates placenta to make Prostaglandins Stimulate more contractions of uterus Positive feedback

67. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Birth, or parturition, is brought about by a series of strong, rhythmic uterine contractions

68. LE 46-19 Dilation of the cervix Expulsion: delivery of the infant Placenta Cervix Uterus Umbilical cord Delivery of the placenta Uterus Placenta (detaching) Umbilical cord

69. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The Mother’s Immune Tolerance of the Embryo and Fetus A woman’s acceptance of her “foreign” offspring is not fully understood It may be due to suppression of the immune response in her uterus

70. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Contraception and Abortion Contraception, the deliberate prevention of pregnancy, can be achieved in a number of ways Some general contraceptive methods: – Preventing release of eggs and sperm – Keeping sperm and egg apart – Preventing implantation of an embryo Abortion is the termination of a pregnancy

71. LE 46-20 MaleFemale Method Event Method Vasectomy Production of viable sperm Production of viable oocytes Sperm transport down male duct system Ovulation Sperm deposited in vagina Capture of the oocyte by the oviduct Condom Abstinence Coitus interruptus (very high failure rate) Abstinence Sperm movement through female reproductive tract Transport of oocyte in oviduct Tubal ligation Spermicides; diaphragm; cervical cap; progestin alone (minipill, implant, or injection) Meeting of sperm and oocyte in oviduct Union of sperm and egg Implantation of blastocyst in properly prepared endometrium Morning-after pill (MAP) Progestin alone Birth Combination birth control pill (or injection, patch, or vaginal ring)

72. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Modern Reproductive Technology Recent advances are addressing reproductive problems Amniocentesis and chorionic villus sampling are invasive techniques in which amniotic fluid or fetal cells are obtained for genetic analysis Noninvasive procedures usually use ultrasound imaging to detect fetal condition Modern technology can help infertile couples by in vitro fertilization

73. LE 46-21 Head Body Head Body

74. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Video: Ultrasound of Human Fetus 2 Video: Ultrasound of Human Fetus 2 Video: Ultrasound of Human Fetus 1 Video: Ultrasound of Human Fetus 1