1. The New Breakthrough Appetite Suppressant

2. Presentation Obesity Mechanism - Appetite Suppression Trial PinnoThin™ - Results Recommendations Claims

3. Weight Management Market In July 2003, NBJ cited the Weight Loss segment as one of the largest condition markets in the U.S. with expected market growth at 4.7% Appetite suppressants account for 13% of the US slimming aids market

4. Weight Management Market

5. WHO-International Obesity Task Force, 2005 European National Obesity Rates

6. WHO-International Obesity Task Force, 2005 Prevalence of Obesity across the World

7. Obesity Health Risks Estimates of obesity related costs up to 8% of overall health budget* Obesity Athero- sclerosis Type 2 diabetes Hyper- tension Heart Failure Stroke Kidney Failure Gallbladder disease Cancer * WHO-International Obesity Task Force 2004

8. Weight Management Appetite Suppression

9. Where does PinnoThin™ come from? Over 144 pine trees exist PinnoThin™ comes from the Korean pine tree (Pinus koraiensis) Pinus koraiensis grows in Korea, Japan, Siberia and Manchouria (North of China) PinnoThin™ contains pinolenic acid Pinolenic acid – LCPUFA – Omega-6: C18:3 - 5,9,12 (all cis) CH 3 COOH 5 912

10. Korean Pine Italian Stone Pine Importance of Pine Nut Oil Origin Pine NutsKorean PineItalian Stone PineUnit Pinolenic acid150.35%

11. Regulation of Digestion and Absorption of Food Regulated by Hormonal system Nervous system Reasons for meal termination: Fullness or absence of hunger Decline in pleasantness or reward value of the food being eaten Graaf, de et al., 2004

12. Meal Termination: Depends on: Short-term signals such as stomach distension and on gut hormones like cholecystokinin (CCK) and glucagon- like peptide 1 (GLP1) Sensitivity to these short-term signals is affected by signals that work in the long term like leptin and grhelin Graaf, de et al., 2004

13. Appetite controllers CCK: cholecystokinin GLP1: glucagon-like peptide 1

14. Cholecystokinin (CCK) Small intestinal hormone (first part) Reduces appetite and food intake Determines meal size or meal termination (satiation) Released upon fat or protein intake Slows down gastric emptying

15. Cholecystokinin (CCK) CCK suppresses appetite, and higher amounts of CCK produce larger appetite-suppressing effects Fatty acids > C10 are effective in releasing CCK Long chain fatty acids are more effective than medium chain fatty acids Poly unsaturated fatty acids are more effective than mono-unsaturated fatty acids Burton-Freeman et al., 2004 Burton-Freeman et al., Degen et al., 2001 Gutzwiller et al., 2004 McLaughlin et al., 1999 Matzinger et al., 2000 Lawton et al., 2000

16. Glucagon-like peptide 1 (GLP1) Small intestinal hormone (last part) Reduces appetite and food intake Determines meal size or meal termination (satiation) Released upon fat and carbohydrates intake Delays gastric emptying

17. Gut responds to Fatty Acids by producing CCK

18. In Vitro Activity of PinnoThin™ Low dose: 100 uM High dose: 300 uM

19. Lipid Nutrition Human Trial at TNO, 2005 Randomized, double blinded, placebo-controlled, cross-over trial Women n=18 (all women tested placebo and PinnoThin™) BMI = 25 – 30 (mean BMI = 27.4 ± 1.6) Age = 55 ± 4 Weight = 76.7 ± 6.7 Overnight fasting Simple breakfast + 3 g of supplement in capsules: PinnoThin™ or Placebo (olive oil) Subjects were monitored during 4 hours: Satiety score (VAS) (subjective) CCK and GLP1 levels (objective)

20. PinnoThin™ Placebo CCK is increased by PinnoThin™ * * * * * = significant difference Cholecystokinin (CCK)

21. CCK level after PinnoThin™ 60% > placebo (P < 0.0001) Total CCK in 4 hours after Supplementation =Area Under the Curve

22. GLP1 is increased by PinnoThin™ PinnoThin™ Placebo * * * = significant difference Glucagon like peptide 1 (GLP1)

23. GLP1 level after PinnoThin™ 25% > placebo (P<0.05) Total GLP1 in 4 hours after Supplementation AUC = Area Under the Curve

24. Summary of the results PinnoThin™ significantly increases CCK and GLP1 in blood within 30-60 min Total CCK and GLP1 levels remain higher than placebo for at least 3 hours after PinnoThin™ intake Desire to eat and the prospective intake scores are significantly lower after 30 min PinnoThin™ reduces the desire to eat with 29% at 30 minutes relative to placebo PinnoThin™ reduces the prospective food intake with 36% at 30 minutes relative to placebo Conclusion PinnoThin™ may thus affect meal size

25. Recommendations To reduce meal size 3 g PinnoThin™ 30 – 60 minutes before a meal Intake ideally before meal with highest energy content To reduce snacking 3 g PinnoThin™ in between meals Maximal daily dose is 6 g per day

26. Safety of 6 g / day History of use in humans Animal studies Ames-test using Pine Nut Oil Clearance from Covance (The Development Services Company)

27. PinnoThin™ is available in Triglyceride (TG) form Applications As supplement in TG form In food as TG form Beverages, bars, dressings etc Applications

28. Regulatory & Patents Regulatory EU: not a Novel Ingredient because of history of use US: PinnoThin™ TG is GRAS PinnoThin™ FFA - NDI submission will be filed, expectations for allowance end 2005 Patents PinnoThin™ is protected for its specific uses in food, food supplements and pharmaceuticals Patent PinnoThin™ as health component (EP 1088552 US6479070 JP 300280/00 KR 10200057291) Patent on composition (EP 1088552)

29. LN’s Patented Claims include: PinnoThin™ promotes the feeling of satiety PinnoThin™ suppresses the desire to eat PinnoThin™ reduces the prospective food intake PinnoThin™ significantly increases the release of CCK PinnoThin™ significantly increases the release of GLP1

30. Examples Consumer Claims PinnoThin™ is an effective appetite suppressant * PinnoThin™ can help to reduce your caloric intake PinnoThin™ can help to reduce snacking PinnoThin™ supports to reduce the food intake PinnoThin™ can control your weight PinnoThin™ gives you control on your appetite * Structure-function claim FDA These claims are not approved by the FDA and the use of these claims depend on the regulations in your country

31. Why PinnoThin™? PinnoThin™ is a scientifically proven appetite suppressant PinnoThin™ is derived from a natural (vegetable) source PinnoThin™ contains the effective components PinnoThin™ is easy to use PinnoThin™ can be taken every day

32. The New Breakthrough Appetite Suppressant www.lipidnutrition.com