1. Bellringer-December 3, 2013
Is genetic engineering a new technology (last 25 years)? Around when do think genetic engineering started?
Have you eaten GMOs?
What crops do you think have the highest likelihood of being GMOs?

2. Genetic Modification Two Methods
Selective Breeding (breeding plants or animals based on selecting desirable characteristics of the parent)
Recombinant DNA (genetic engineering)  GMO
Genetic Engineers can change the DNA code (genes) of living organisms.

3. Natural Selection vs. Artificial Selection
In artificial selection (selective breeding),
humans modify other species over many generations by selecting and breeding individuals with desired traits

5. Selective Breeding
Breed only those plants or animals with desirable traits
People have been using selective breeding for thousands of years with farm crops and domesticated animals.
Think about the zea mays

6. Selective Breeding
The evolution of corn or maize (Zea mays) involved the gradual increase in the size of the grain head (ear) shown here, as well as the development of varieties that would tolerate the prevailing climatic conditions in different areas of the New World.

8. How does it work?
In cross pollination (think Mendel’s peas), we are combining two traits to get a mixture of results
In genetic engineering, a single gene, a half page recipe in the 52-thousand-page set of recipe books, can direct the plant to make new traits or remove them

9. Genetic Engineering- Recombinant DNA
The ability to combine the DNA of one organism with the DNA of another organism. This is where genes from one species can be introduced into an entirely different species through human intervention.
An organism that is generated through genetic engineering is considered to be a genetically modified organism (GMO).
Recombinant DNA technology was first used in the 1970’s with bacteria.

10. History?
The first GMOs were bacteria in 1973; GM mice were generated in 1974. Insulin-producing bacteria were commercialized in 1982 and genetically modified food has been sold since Glofish, the first GMO designed as a pet, was first sold in the United States December in 2003.

11. Recombinant Bacteria Remove bacterial DNA (plasmid).
Cut the Bacterial DNA with “restriction enzymes”.
Cut the DNA from another organism with the SAME “restriction enzymes”.
Combine the cut pieces of DNA together with another enzyme and insert them into bacteria.
Reproduce the recombinant bacteria.
The foreign genes will be expressed in the bacteria.

12. How to Create a Genetically Modified Plant
1.Create recombinant bacteria with desired gene.
2. Allow the bacteria to “infect" the plant cells.
3. Desired gene is inserted into plant chromosomes.

13. Figure 20.2
Bacterium
Bacterial chromosome
Plasmid
Cell containing gene of interest
Recombinant DNA (plasmid)
Gene of interest
DNA of chromosome
Recombinant bacterium
Protein harvested
Basic research on protein
Copies of gene
Basic research on gene
Gene for pest resistance inserted into plants
Gene used to alter bacteria for cleaning up toxic waste
Protein dissolves blood clots in heart attack therapy
Human growth hormone treats stunted growth
Protein expressed by gene of interest 3
Overview of gene cloning with a bacterial plasmid, showing various uses of cloned genes

15. Transgenic
If genetic material from another species is added to the host, the resulting organism is called transgenic.
Trangenic organisms are a subset of GMOS. They are a subset of GMOs because they are the species in which their DNA is inserted into another species.

16. Examples
For example, taking a gene from a jellyfish and inserting into the zebrafish genome created the “glofish” – fish that glow in the dark which are now being sold on the market.
Another example is genes from a bacteria inserted into food crops so that the crop can create it’s own insecticide. These plants and animals are also known as “transgenic”.

18. How to Create a Transgenic Animal
Desired DNA is
added to an egg cell.

19. The DNA of plants and animals is altered for several reasons
Disease-resistant
Insect-resistant crops
Hardier and bigger fruit
Increased vitamin content
“Pharming” purposes (ex. Vaccine)
70-75% of food in supermarket is genetically modified.

20. What are Genetically Modified Foods?
(“Frankenfoods”?)
You have already eaten GM foods. Some GM tomatoes, for example, have had their genes altered to stop them from going soft while they are still growing. For several years they were widely sold in tomato paste.
The GM foods we eat have all been tested for safety. But some people worry about the long term effects of eating genetically modified foods!

21. What Have I Eaten?
Genetically modified (GM) foods possess specific traits such as tolerance to herbicides or resistance to insects or viruses.

22. Likely GMO Crops Alfalfa (first planting 2011)
Canola (approx. 90% of U.S. crop)
Corn (approx. 88% of U.S. crop in 2011)
Cotton (approx. 90% of U.S. crop in 2011)
Papaya (most of Hawaiian crop; approximately 988 acres)
Soy (approx. 94% of U.S. crop in 2011)
Sugar Beets (approx. 95% of U.S. crop in 2010)
Zucchini and Yellow Summer Squash (approx. 25,000 acres)
ALSO high-risk: animal products (milk, meat, eggs, honey, etc.) because of contamination in feed.
1) Type of flowering plant in the pea family

23. Can We End World Hunger? Malnutrition and vitamin deficiencies?
Golden rice is genetically modified rice that now contains a large amount of A-vitamins. Or more correctly, the rice contains the element beta-carotene which is converted in the body into Vitamin-A. So when you eat golden rice, you get more vitamin A.
Golden rice
Beta-carotene gives carrots their orange color and is the reason why genetically modified rice is golden. For the golden rice to make beta-carotene three new genes are implanted: two from daffodils and the third from a bacterium
Advantages:
The rice can be considered a particular advantage to poor people in underdeveloped countries. They eat only an extremely limited diet lacking in the essential bodily vitamins. The consequences of this restricted diet causes many people to die or become blind. This is particularly true in areas of Asia, where most of the population live on rice from morning to evening.

24. Going Bananas?
According to recent reports, the world may soon be out of bananas.
Bananas are propagated through asexual cloning, so all bananas are genetically identical
Banana plantations in Africa, Asia and Central America are uniquely susceptible to fungi, viruses and pests.
Unless scientists can find a way to genetically enhance the banana’s ability to ward off parasites, we could be bananaless in ten years.
Several agroscience companies believe they can genetically engineer such an invincible banana by copying parts of the genetic codes of other fruits and instilling them into the banana.

25. Genetically modified organisms are called transgenic organisms.
TRANSGENIC ANIMALS
Fish – grow faster than normal aquaculture salmon
Chickens – more resistant to infections
Cows – increase milk supply and leaner meat
4. Goats, sheep and pigs – produce human proteins in their milk

26. Transgenic Goat
This goat contains a human gene that codes for a blood clotting agent. The blood clotting agent can be harvested in the goat’s milk.
Human DNA in a Goat Cell .

27. Transgenic Fish
AquAdvantage salmon grow twice as fast as wild Atlantic salmon.
Food and Drug Administration (FDA) is in the final stages of deciding whether to allow GM salmon on to the market.
Aldi and Whole Foods among retailers refusing to sell product
Researchers are also working to bring GM cows, chickens and pigs to market.

29. Are they Safe?
The GM products that are currently on the international market have all passed risk assessments conducted by national authorities.
These different assessments include an assessment of environmental and human health risk.
These assessments are thorough, they have not indicated any risk to human health.

30. Is it safe to eat? Why isn't it labeled in the U.S.?
The U.S. government's position: Genetically engineered crops are safe, resist disease better, and can provide much-needed food in starving nations.
The EU position: Keep it out. We prefer organic, which is much healthier. The risk of genetically modified foods to health and the environment outweigh the benefits. Only the multinational biotech companies will benefit, dominating the world food supply and squeezing out traditional farmers.
The U.S. is the largest producer of genetically modified crops.

31. 'Frankenfood' Fears
Experts say this science, like any other, has no guarantees. Risks include:
Introducing allergens and toxins to food
Accidental contamination between genetically modified and non-genetically modified foods
Antibiotic resistance
Adversely changing the nutrient content of a crop
Creation of "super" weeds and other environmental risks
Benefits include:
Increased pest and disease resistance
Drought tolerance
Increased food supply

32. Monsanto
Monsanto Company is a publicly traded American multinational chemical, and agricultural biotechnology corporation headquartered in Creve Coeur, Missouri.
It is a leading producer of genetically engineered (GE) seed and of the herbicide glyphosate, which it markets under the Roundup brand.
Monsanto was among the first to genetically modify a plant cell, along with three academic teams, which was announced in 1983, and was among the first to conduct field trials of genetically modified crops, which it did in 1987

33. Consumer Attitudes
Many consumers more readily accept biotechnology as beneficial for their health (e.g. medicines with improved treatment potential).
In the case of the first GM foods introduced onto the European market, consumers felt the products were of no apparent direct benefit to (not cheaper, no increased shelf-life, no better taste).
Consumers have questioned the validity of risk assessments, both with regard to consumer health and environmental risks, focusing in particular on long-term effects.
Other topics for debate by consumer organizations have included allergenicity and antimicrobial resistance.
Consumer concerns have triggered a discussion on the desirability of labelling GM foods, allowing an informed choice. At the same time, it has proved difficult to detect traces of GMOs in foods: this means that very low concentrations often cannot be detected.
In the late 1980s – early 1990s, the results of decades of molecular research reached the public domain. Until that time, consumers were generally not very aware of the potential of this research. In the case of food, consumers started to wonder about safety because they perceive that modern biotechnology is leading to the creation of new species.
Consumer confidence in the safety of food supplies in Europe has decreased significantly as a result of a number of food scares that took place in the second half of the 1990s that are unrelated to GM foods. This has also had an impact on discussions about the acceptability of GM foods.

35. Pros and Cons
Safety
Potential human health impact: allergens, transfer of antibiotic resistance markers, unknown effects
Potential environmental impact: unintended transfer of transgenes through cross-pollination, loss of flora and fauna biodiversity
Access and Intellectual Property
Domination of world food production by a few companies
Increasing dependence on Industralized nations by developing countries
Ethics
Violation of natural organisms' intrinsic values
Tampering with nature by mixing genes among species
Objections to consuming animal genes in plants and vice versa
Stress for animal
Labeling
Not mandatory in some countries (e.g., U. States)
Mixing GM crops with non-GM confounds labeling attempts
Crops
Better taste and quality
Less time to ripen.
More nutrients, more food, and stress tolerance
Improved resistance to disease, pests, and herbicides
New products and growing techniques
Animals
Increased resistance, productivity, hardiness, and feed efficiency
Better yields of meat, eggs, and milk
Improved animal health and diagnostic methods
Environment
"Friendly" bioherbicides and bioinsecticides
Conservation of soil, water, and energy
Better natural waste management
More efficient processing
Society
More food for growing populations

36. Bizarre examples Glow in the dark cats Venomous Cabbage
(scorpion poison)

37. Exit Slip What the two methods of genetic modification?
What are two reasons GMOs are engineered?
Name and explain one example of the positive effects of GMOs.
Name at least two likely GMO crops.
What two areas of risk do the risk assessment assess?