1. BRAVO: Superior Design for Handheld Raman January 28th, 20151 Yan Wang, Ph.D. Presentation at IFPAC Summit PR 2015

2. 2 BRAVO Handheld Raman Spectrometer Highlights at a Glance January 28th, 2015  LASER class 1M product  SSE TM – Sequentially Shifted Excitation (Patented)  Duo LASER TM excitation  IntelliTip TM - automated measuring tip recognition  Intuitive and guided touchscreen operation  Automated batch scan reporting  Inbuilt wavenumber calibration  Robust and precise optics  Wireless data exchange  Complies with 21 CFR Part 11 requirements

3. 3 BRAVO Handheld Raman Spectrometer Hardware January 28th, 2015 Barcode button activates 2D barcode reader Laser release button On/off button BRAVO Battery door Keypad Laser on/off button Measuring tip (bag tip shown)

4. 4 Sequentially shifted excitation – SSE TM Mitigate Fluorescence January 28th, 2015 J.B. Cooper, M. Abdelkader, K.L. Wise, Appl. Spectros. 67, 973–984 (2013). J.B. Cooper, S. Marshall, R. Jones, M. Abdelkader, K.L. Wise, Appl. Optics. 53, 3333–3340 (2014). SSE TM (patented) method generates Raman spectra in true spectral space  does not require generation (and subsequent reconstruction) of derivative spectra (SERDS)  results in improved signal-to-noise ratio compared to traditional fluorescence rejection methods while providing instrument-limited bandwidth resolution 50010001500200025003000 Raman Shift / cm-1 Raman Intensity  -Lactose monohydrate No impact of Fluorescence

5. 5 Sequentially shifted excitation – SSE TM Mitigate Fluorescence January 28th, 2015 40060080010001200140016001800 Raman shift cm-1 Raman Intensity Talc in PE BRAVO convenient dispersive Raman spectrometer

6. 6 Duo LASER TM Access the CH stretching region January 28th, 2015 50010001500200025003000 Raman Shift / cm-1 Raman Intensity 4-Acetamidophenol FT-Raman (spectral resolution 8cm -1 ) BRAVO Access to the CH stretching range avoids erroneous verification/identification Examples: Discrimination between different polymorphs of a drug Contamination of sample with e.g. cyanide (cannot be observed if the Raman shifts ends at 1800cm -1 )

7. 7 IntelliTip TM Always use the proper measuring tip January 28th, 2015 Inner Spacer Bag Cap IntelliTip TM  The locking mechanism design guarantees quick and highly reproducible connection/ disconnection of measuring tips.  ID chips prevent to release the Laser unless a tip is connected. Even more, if a measuring tip is defined in the library for a chemical material, BRAVO will inform the user which measurement tip needs to be used for the verification.

8. 8 Intuitive, gesture-based touch screen GUI Speed up your workflow January 28th, 2015 Validity of Performance Qualification (PQ) and Operational Qualification (OQ) Drop down menu lists messages, warnings, etc. indicated by the envelope icon on the upper left Network connectivity Battery lifetime General Information

9. 9 Intuitive, gesture-based touch screen GUI Login Screen January 28th, 2015 Enter Username:  hit any letter on the onscreen keyboard (OSK) that is contained in the name you like to enter Example:  hit A and get a list of all names containing A  select the proper entry, or scroll down to find the proper entry, or hit letter next to A to shorten the selection list Enter Password:  Enter password on OSK The approach described above will not work!

10. 10 Intuitive, gesture-based touch screen GUI Main Screen January 28th, 2015 Sample Verification  will guide through the verification procedure and ends up with an Accepted / Rejected information Acquire Materials  can be used to acquire spectra for building a library Review Results  allows to review formerly measured spectra still being stored on the BRAVO Library Management  allows to select/deselect library for verification Settings  allows for basic settings like date, time, LCD brightness, etc. (availability depends on user rights)

11. 11 Intuitive, gesture-based touch screen GUI Sample Verification Workflow January 28th, 2015 Material Name: Describes the name of a chemical compound to be analyzed in its packaging (example: Talc in PE bag, Talc in vial), this is a required entry Batch/Lot# and Container# can be filled in but are not mandatory for proceeding  All information can be entered via the OSK in the above explained manner or by using the 2D barcode reader which can either be activated on the touchscreen by touching the following icon or by pressing the barcode button on the BRAVO keypad Start sample verification workflow by touching sample verification icon Operator

12. 12 Intuitive, gesture-based touch screen GUI Sample Verification Workflow January 28th, 2015 Once the sample information are completed  Touch the Laser icon or swipe with the finger in direction of the arrows Operator

13. 13 Intuitive, gesture-based touch screen GUI Sample Verification Workflow January 28th, 2015  IntelliTip TM now tells which measuring tip needs to be used To start the verification:  touch the Press to Start icon or press Laser release button on the BRAVO keypad Place Vial into Sample Holder Operator

14. 14 Intuitive, gesture-based touch screen GUI Sample Verification Workflow January 28th, 2015 Results  it’s clearly visible whether the verification was Accepted or Rejected  derived spectrum and library spectrum can be viewed on Results screen  touching the barcode icon brings back to the first screen  in case of entries on the first screen differ by Container # only, a message will appear stating how many containers had been measured already Operator Batch Scan  Touching this icon brings back to the first screen. Material Name and Batch/Lot # are already filled in. Results screen

15. www.bruker.com © Copyright Bruker Corporation. All rights reserved.