1. User Interfaces for University Laboratory Work cells Malcolm Crook Process Analysis & Automation Lecturer, MChem Course, University of Surrey Lecturer, MSc Course Trinity College Dublin Advisory Board, University of Manchester

2. Purpose of presentation to show … Software options – Vendor specific – Vendor independent Hardware options – Usually decided by user Systems integration – Putting the system together Tailoring projects – Making the system useable

3. analyze sampleprepare sample analyze sample prepare sample Laboratory automation - the process collect sample report results

4. Key software requirements Run a batch of plates Run another batch of plates if present Reliable Easy to use

5. Software Choices Time driven scheduler – Unsuitable for the tasks suggested in the documents Event driven scheduler – Can handle timings – Can handle decisions – Can handle unknown plate quantities at the beginning of the run Overlord2 is an EVENT DRIVEN SCHEDULER Overlord Scheduler is a TIME RESOLVED SCHEDULER

6. control and scheduling software STATIC hit the GO button know exactly what will happen at any time in the process DYNAMIC hit the GO button Follow a set process

7. control software - summary real-time scheduling – OVERLORD Workstation – event driven time-resolved scheduling – OVERLORD Scheduler – time driven Either

8. common features support for over 300 instruments data handling facilities utilizes original instrument software can integrate with most databases – SQL server, oracle, access 21 CFR part 11 compliant full system tracking error notification error recovery

9. which product ? AdvantagesDisadvantages Very Flexible True Dynamic Scheduling Takes longer to setup Very easy to use Obeys critical timings Schedules fixed processes Cannot make decisions Overlord2 OVERLORD Scheduler

10. Overlord2 dynamic/real-time/event icon based drag and drop set-up flow chart interface very flexible dynamic scheduling – decisions can be made in real-time

11. Software Structure Instrument Interface Run native instrument control software Instrument Interface

12. Software Structure Overlord2 Driver Instrument Interface Run native instrument control software Overlord2 Driver Written to access key functionality Overlord2 Driver Instrument Interface

13. Supervisor layer Software Structure Overlord2 Overlord2 Driver Instrument Interface Run native instrument control software Overlord2 Driver Written to access key functionality Event driven Overlord2 Driver Instrument Interface

14. Overlord2

15. which product ? AdvantagesDisadvantages Very Flexible True Dynamic Scheduling Takes longer to setup Very easy to use Obeys critical timings Schedules fixed processes Cannot make decisions Overlord2 OVERLORD Scheduler

16. static/time driven icon based drag and drop Gantt chart interface optimise and interleave different assays e ach assay run with exact incubation timings very easy to create an assay add new assays when the system is running

17. OVERLORD Scheduler

18. HARDWARE OPTIONS

19. Most manufacturers Most instruments Over 300 instruments 450 licenses Used in – USA – Europe – Japan – Australia

20. HARDWARE OPTIONS Plate transport

21. Plate transfer choice Stackers Hudson PlateSilo Magstar Stacker PerkinElmer LS PlateStak Titertek Titan Thermo RapidStak plate stacker Velocity 11 BenchCel Stacker Robots automate.it KiNEDx SCARA arm Caliper (Zymark) Twister I & II Denso VS-6556E robot Hamilton Microlab SWAP Hudson Plate Crane Mitsubishi robots PAA TUBEstar / WELLstar / STORstar PAA X-Link Sands Robot R16 Staubli robots Thermo CataLyst 5 & X

22. HARDWARE OPTIONS – stackers PE PlateStakThermo RapidStak

23. HARDWARE OPTIONS - robots

24. automate.it SCARA HARDWARE OPTIONS - robots PE PlateStakMitsubishi

25. HARDWARE OPTIONS Peripheral equipment

26. HARDWARE OPTIONS- peripheral equipment Plate manipulatorHigh content plate reader Peripherals

27. HARDWARE OPTIONS- peripheral equipment Reagent dispensing Flexdrop Liquid Handling PlateWash

28. HARDWARE OPTIONS- peripheral equipment Barcode reader Plate reader

29. HARDWARE OPTIONS Storage/containment

30. HARDWARE OPTIONS - storage Inheco 12 place incubator Thermo Cytomat range of incubators Liconic range of incubators Hamilton (TekCel) PlateStore Thermo (Jouan) MolBank Incubator Caliper Scitec Carousel Storage

31. HARDWARE OPTIONS - containment Bench workcell automate.it SAFEcel automate.it SAFEcel L automate.it SAFEcel C2 automate.it connect 2 robot solution

32. Systems Integration

33. what is an integrator ? instruments are BRICKS software is the CEMENT integrator puts it together like a BUILDER hardware softwareintegration system

34. example systems No stacker Stacker Robot Tracked robot

35. no stacker or laboratory robot PerkinElmer JANUS system http://www.paa.co.uk/ftp/labauto/videos/elisa_jpm_lo.wmv

37. stacker only automated TTP Mosquito system http://www.paa.co.uk/ftp/labauto/videos/nano_mosquito_lo.wmv automated PerkinElmer PlateStak system http://www.paa.co.uk/ftp/labauto/videos/elisa_pe_lo.wmv

38. laboratory robot systems automated Innovadyne Nanodrop system http://www.paa.co.uk/ftp/labauto/videos/nano_innovadyne_lo.wmv automated GE Healthcare IN Cell 1000 system http://www.paa.co.uk/ftp/labauto/videos/hcs_1000_lo.wmv

39. factory robot systems automated dilute & seal system http://www.paa.co.uk/ftp/labauto/videos/mitsubishi_dilute_seal_lo.wmv automated cell system http://www.paa.co.uk/ftp/labauto/videos/ppw_lo.wmv

40. tracked robot system BSE workcell http://www.paa.co.uk/ftp/labauto/videos/milk_lo.wmv

41. Tailoring systems

42. Overlord2 - Customizable user GUI & runtime module separable can use different GUI, Excel could be used different run engines can be run processes the flow using Overlord variables overlord instrument drivers developed quickly all components can be modified master or slave all errors handled with different options.NET framework, API will be utilized flexible GUI makes writing assays easier

43. User layer Software Structure Experiment Manager Overlord2 Overlord2 Driver Instrument Interface Run native instrument control software Overlord2 Driver Written to access key functionality Event driven Overlord2 Driver Instrument Interface

44. Experiment Manager – User layer Add an experiment Experiments added Experiment runningExperiment complete

45. External collaborations Overlord2 GE: Automation front ends Molecular Devices StakMax Labcyte 810 Pod: Automation layer Caliper ACES programme TAP: Automation layer Matrical automated workcell OVERLORD Scheduler PerkinElmer: JANUS Project Manager

46. conclusion laboratory automation has a number of key stages choice – hardware – software scheduler choice User front end choice – integration make the system what was required not what was asked for committed to technically relevant cost effective solution