1. Safe handling of enzymes
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Safe handling of enzymes
17/04/2017

2. AGENDA WHAT ARE ENZYMES? POTENTIAL RISKS OF ENZYME EXPOSURE
4/17/2017
NOVOZYMES PRESENTATION
AGENDA
WHAT ARE ENZYMES?
POTENTIAL RISKS OF ENZYME EXPOSURE
REDUCTION OF ENZYME EXPOSURE

3. What are Enzymes? Special kind of proteins found in all living matter.
17/04/2017
NOVOZYMES PRESENTATION
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What are Enzymes?
Special kind of proteins found in all living matter.
Vital for life, but not living substances themselves
Catalysts - accelerate chemical reactions
Specific and efficient in very small amounts
In laundry detergents they break up stains so they can be washed away more easily

4. AGENDA WHAT ARE ENZYMES? POTENTIAL RISKS OF ENZYME EXPOSURE
4/17/2017
NOVOZYMES PRESENTATION
AGENDA
WHAT ARE ENZYMES?
POTENTIAL RISKS OF ENZYME EXPOSURE
REDUCTION OF ENZYME EXPOSURE

5. Potential Adverse Effects of Enzymes
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Potential Adverse Effects of Enzymes
Primary Irritation (eyes, skin, mucous membranes)
Proteases
Allergy
Only through inhalation (not through skin contact)
Like allergy to other soluble proteins (pollen, mold, animal dander)
INDUSTRIAL ENZYMES ARE NOT TOXIC

6. Prevention of Skin and Eye Irritation
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Prevention of Skin and Eye Irritation
Keep high personal hygiene standard
Avoid direct contact with enzymes
Use personal protective equipment
Moisture increases irritation
Goggles and face shields
Gloves
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NOVOZYMES A/S

7. Main Causes of Allergy Development
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Main Causes of Allergy Development
Exposure pattern
Individual predisposition
Physical form of the allergen
Genetically (“atopics”)
Smoking habits
Particle size
Level
Time
Frequency
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Allergens
Allergens (e.g. pollen, house dust mites, molds, hair from dog/cat, etc. ) are proteins.
Proteins in the form of dust may cause allergy when inhaled.
Enzymes are proteins.
Enzymes may therefore also cause allergy when inhaled.

9. NOVOZYMES PRESENTATION
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Allergy Development
ALLERGENS
DUST CONTROL
SENSITIZATION
MEDICAL SURVAILLANCE
SYMPTOMS
NOVOZYMES PRESENTATION
NOVOZYMES A/S

10. Dust Control Personal protection Equipment Personal Hygiene Training
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Dust Control
Personal protection
Equipment
Face masks, Respirators
Goggles, Gloves
Full Body Protection
Personal Hygiene
Shower
Clothing
Training
Procedures
Allergy Information

11. Sensitization vs. Allergy
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Sensitization vs. Allergy
Sensitization
Your body makes specific antibodies against the enzyme
No symptoms
Allergy
After repeated enzyme exposure
Antibodies cause symptoms
Symptoms are immediate or delayed
Symptoms are reversible
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12. Allergy Sensitization:
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Allergy
Sensitization:
A state where a person has developed specific antibodies to an allergen, e.g. pollen, flour, etc…
Can be detected by “Skin Prick Test” or RAST
Without symptoms is not a disease
Is induced by inhalation of allergens, e.g. dust
Sensitization is lifelong!
DEFINITIONS AND CLARIFI-CATIONS

13. Allergy Allergy: Some sensitized people develop allergy symptoms
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Allergy
Allergy:
Some sensitized people develop allergy symptoms
Asthma or Hay-Fever
Symptoms disappear when out of exposure
Symptoms reoccur when re-exposed
Enzyme allergy is a occupational disease
DEFINITIONS AND CLARIFI-CATIONS

14. Allergy symptoms Itching & irritation of eyes Sneezing
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Allergy symptoms
Itching & irritation of eyes
Sneezing
Nasal or sinus congestion
Coughing
Tightness of chest
Hoarseness
Shortness of breath
Asthma

15. AGENDA WHAT ARE ENZYMES? POTENTIAL RISKS OF ENZYME EXPOSURE
4/17/2017
NOVOZYMES PRESENTATION
AGENDA
WHAT ARE ENZYMES?
POTENTIAL RISKS OF ENZYME EXPOSURE
REDUCTION OF ENZYME EXPOSURE

16. Enzyme Safety Program Goal Elements of the program
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Enzyme Safety Program
Goal
To prevent sensitization and allergic reactions by controlling inhalation of dust and aerosols
Elements of the program
Enzyme product formulation
Engineering control
Good work practices

17. Enzyme Product Formulation
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NOVOZYMES PRESENTATION
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Enzyme Product Formulation
Liquids
Powders
Granulates (encapsulated/ coated enzymes)
Very high potential for dust generation
Use of powders should be avoided
Low dust generation
Potential dust generation from crushed granulates
Potential aerosol generation
Potential dust generation from dried spills
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Engineering Control
Systems, processes, or equipment that reduce dust or aerosols at the source
The primary way that dust and aerosols are controlled
Tell your supervisor/safety officer of any ideas for improving the control of dust and aerosols

19. Types of Engineering Controls Contained handling systems
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Engineering Control
Types of Engineering Controls
Contained handling systems
Avoid conveyors that may break up enzymes
Enclosures
Enclose operations such as filling stations that have the potential to generate dust or aerosols
Local exhaust ventilation
Use for operators where dust and aerosols are unavoidable

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NOVOZYMES PRESENTATION
Good Work Practices 1
Avoid direct contact with enzymes and enzyme contamination on clothes
Avoid generation of dust or aerosols
Remove spills immediately
No sweeping or high pressure washing
Vacuum only with HEPA filters
Moisten to remove completely

21. 4/17/2017
NOVOZYMES PRESENTATION
Good Work Practices 2
Wear personal protection when needed (exposure can’t be eliminated)
Keep high personal hygiene standard
Wash hands often
Change work clothes frequently
A shower is recommended before going home

22. 4/17/2017
NOVOZYMES PRESENTATION
Good Work Practices 3
Good work practices are of special importance around high risk operations
Addition of enzymes to the process, i.e. spray delivery systems
Mixing/Blending
Handling empty containers
Maintenance of ventilation systems

23. Air and Medical Monitoring
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NOVOZYMES PRESENTATION
Air and Medical Monitoring
Air sampling is conducted to asses the levels of enzyme aerosols (dusts or mists)
Looking for levels to be under 60ng/m3
Utilize high-volume air samplers to collect aerosols on a filter for analysis
High levels may result in changes to equipment, engineering controls, or PPE for exposed employees
Medical monitoring looks for early signs of problems
Includes skin prick/RAST testing and lung function testing

24. SUMMARY Enzymes are NOT toxic.
17/04/2017
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SUMMARY
Enzymes are NOT toxic.
Like other proteins (molds, pollen, animal etc..) enzymes may cause allergic reactions in susceptible persons.
Allergy symptoms occur in susceptible persons via inhalation of fine air-borne enzyme dust (< 50 micron).
Irritation (eyes, skin, mucous membranes) is induced by protease only.

25. Safety First! Primary Safety Goal:
17/04/2017
NOVOZYMES PRESENTATION
Safety First!
Primary Safety Goal:
Avoid generation and inhalation of dust or aerosols
This can be achieved by:
Enzyme product design
Engineering controls
Good Work Practices including Personal Protective Equipment (PPE)

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THANK YOU!