1. Aim: How have microscopes
impacted science?
DO NOW:
Explain this
cartoon in your
own words

2. Microscopes
Microscopy is the art of producing images for microscopic things
that are not visible to the
human eye.

3. Robert Hooke's microscope (1665)

4. The first useful microscope
Microscopes Through Time
The first useful microscope
was developed in the
Netherlands in
the early 1600s

6. Types of Microscopes Compound Light Microscope
Models found in most schools, uses compound lenses and light to magnify objects. The lenses bend or refract the light, which makes the object beneath them appear closer.

7. 2. Stereoscope –
this microscope allows for binocular (two eyes) viewing of larger specimens.

8. Parasite that causes disease in your liver!
Polychaete worm (aquatic)
Parasite that causes disease in your liver!

9. 3. Scanning Electron Microscope –
allow scientists to view a universe too small to be seen with a light microscope. SEMs do not use light waves; they use electrons

10. 4. Transmission Electron Microscope
also uses electrons, but instead of scanning the surface (as with SEM's) electrons are passed through very thin specimens.

11. an Electron microscopes!
Living specimens
can NOT be
viewed under
an Electron microscopes!
WHY?
Due to the powerful laser beam, anything living would die!

12. A mosquito An ant

13. Threaded needle Scales of a
moth wing

14. Antarctic Mite

15. Stinger of a mosquito

16. Aim: How do the parts of the microscope
assist science?
DO NOW:
Hand-out

17. Parts of a Compound light microscope

18. The structure of a cell nucleus would be seen in the greatest detail by use of
a compound light microscope
an ultracentrifuge
a dissecting microscope
an electron microscope

19. Which is the correct sequence of historical developments leading to our present knowledge of cells?
electron microscope – cell theory – compound light microscope
compound light microscope – cell theory – electron microscope
cell theory – electron microscope – compound light microscope
electron microscope – compound light microscope – cell theory

20. Eyepiece/ ocular Bodytube arm stage base nosepiece Stage clips
Objective lens
Objective lens
stage
Objective lens
Stage clips
Coarse adj.knob
Diaphragm
Fine adj. knob
Light source
base

21. Eyepiece (Ocular lens): magnifies the image 10x

22. & Objectives (4x, 10x & 40x magnification)
Stage Clips
& Objectives (4x, 10x & 40x magnification)

23. Objective lens Attached to the nosepiece Usually three magnifications
Examples: 4X, 10X, 40X
Referred to as low and high powers

24. Low power objective =
used to locate the specimen on the slide
Larger field of view (See more of the slide, but less details)
High power objective =
More magnification
Smaller field of view but more details

25. Deer Tick 41X Magnification

26. Deer Tick 657X Magnification

27. Diaphragm Used to adjust the amount of light entering the scope
Located beneath the stage
Diaphragm

28. Coarse (big) and Fine (small) adjustment knobs

29. ARM

30. TO CARRY THE MICROSCOPE!!!
Remember!!
ALWAYS USE TWO HANDS
TO CARRY THE MICROSCOPE!!!

31. 1.)
Diaphragm

32. 2. 3.

33. 4. 5.

34. 5.

35. Which structure is best observed using a compound light microscope?
a cell
a virus
a DNA sequence
the inner surface of a mitochondrion

36. After switching from the high-power to the low-power objective lens of a compound light microscope, the area of the low-power field will appear
larger and brighter
smaller and brighter
larger and darker
smaller and darker

37. Total
Magnification
Is numeric value that quantifies how much a specimen has been magnified.
EX. 450X , 20X , 100X

38. Is calculated by multiplying the eyepiece power (usually 10x) by the objective lens in place.

40. What is the lowest possible magnification that can be obtained using the microscope shown?
20x
200x
40x
800x

41. Total magnification: Ocular X Objective = total
(eyepiece) magnification
Ex.)
Objective
lens
Total magnification 4x
10x
43x

43. When viewed with a compound light microscope under low power, the letter "p" will appear asq p d b

44. How have microscopes impacted science?
THE END