1. Introduction to the compound Microscope

2. Types of Microscopes Compound Microscope Stereoscope or dissecting scope Onion cells (100x) Cheek cells (400x) Two spotted spider mite (20x)

3. Types of Microscopes TEM - Transmission Electron Microscope

4. Types of Microscopes SEM - Scanning Electron Microscope

5. Eye piece Body Tube Revolving nosepiece Objectives Stage Clips Stage Diaphragm Mirror (light source) Base Coarse Adjustment Fine Adjustment Arm

6. Always carry with 2 hands. Place one hand on the arm and one hand on the base. Make sure the microscope is on a flat surface and away from the edge of the table. Always Look to the side when changing objectives and rotate carefully. Always use low (scanning) power first. Always store the microscope covered and with the lowest powered objective in place. Only use lens paper for cleaning Rules for using the microscope

7. Microscope Basics: Determining Total Magnification Multiply the magnification of the ocular lens (eyepiece) by the magnification of the objective lens. 10 x 4 x Total magnification = 10 x 4 = 40 We represent this magnification as 40X

8. Microscope Basics: Field of view Field of view is what you see when you look into a microscope. As you increase magnification your field of view becomes smaller

10. Microscope Basics: Depth of focus. Refers to how much of the specimen you can focus clearly on at one time in your field of view. As you increase magnification your depth of focus becomes narrower and less of your sample can be focused at one time.