1. Distributor Meeting MEDICA November, 2009 Dr. Ariel Weisz R&D Department Metrolab, Argentina

2. Metrolab is a company which designs and manufactures Clinical Chemistry Analyzers with its own technology. + reliable instruments to the global market + easy of use + effective after-sale service + competitive prices

3. Clinical Chemistry Analyzers The Principle: Spectrophotometry

4. Beer-Lambert Law concentration Absorbance T = P / P 0 A = log 10 (P 0 / P) A =  bc  : molar absorbtivity c: molar concentration

5. Colorimetry Samples Reactions Reagents MixIncubation (37°C) Measure

6. Double beam photometer reference detector sample detector beam splitter filter lamp reaction cuvettes A = log(R/S) R S

7. Dual photometer Front sample detector reference detector beam splitters filter lamp A F = log(R/S F ) A B = log(R/S B ) SFSFSFSF R Back sample detector SBSBSBSB

8. Clinical Chemistry Analyzers Applications and Measuring Methods

9. One Reagent End Point A Time Absorbance B R 1 +S Measure = A - B Metabolites Albumin Glucose Cholesterol Uric acid Triglycerides Total proteins

10. A2A2A2A2 Time Absorbance A1A1A1A1 R 1 +S R2R2R2R2 B1B1B1B1 B2B2B2B2 Measure = (A 2 -A 1 ) – (B 2 -B 1 ) Two-Reagent End Point Bilirubin IgA, IgG, IgM LDL, HDL Phosphorous

11. A2A2A2A2 Time Absorbance A1A1A1A1 R 1 +S B2B2B2B2 B1B1B1B1 Measure = (A 2 -A 1 ) – (B 2 -B 1 ) Fixed Point Kinetics ALP Creatinine Fructusamine Urea

12. Kinetics AnAnAnAn Time Absorbance A1A1A1A1 BnBnBnBn B1B1B1B1 R 1 +S Measure = rate A – rate B Enzymes Amylase CK, CPK GGT GOT GPT

13. Clinical Chemistry Analyzers METROLAB Instruments

14. // Metrolab history 4000 1600 DRK 1990199520002005 1600 PLUS21002300 1600 DR 305 315 330 1985

15. // Current Line - Main Instruments medium labsbig labs / hospitalssmall labs M4000 600 test/hr 400 200 M2300v4 M2300GL M1600DRv3 semiautomatic 10-40 patients/day 240 test/hr 20-60+ patients/day 280 test/hr 60-250+ patients/day 600 test/hr veterinary

16. Clinical Chemistry Analyzers METROLAB 4000

17. Troughput: 550 test/hr without ISE 600 test/hr single reagent 450 test/hr double reagent 720 test/hr with ISE

18. Split reagent trays for effective carryover and interference control Methods are distributed, at operator’s will between two trays. In this way, interfering methods never come into contact in the same cuvette

19. Continuous sample load with coded sectors Positions can be assigned to normal samples or STATS, Handling priorities accordingly

20. Positive BCR identification of samples, reagents and sectors Coded sectors for instantaneous load Any coding system for samples Coded reagents and optional closed system Open channel support

21. Optional ISE module: Na +, K +, Cl - Direct serum readings Indirect urine readings (dilution 1:10) Integrated pack for simple operation Automatic electrode conditioning

22. User friendly software with different access levels Menu bar Main operation control tool bar Messages list Requests to operator bar Quick keys menu Current activity and summary Test in progress Reagents status report General status bar

23. Mixer in probe High efficiency mixing guarantees reliable data in double reagent systems and viscous media Shake time can be programmed for every method

24. 72 on board cooled single reagents R2 30 ml R1 40ml Outer 30 ml Full size 70 ml

25. LIS Interface Connection - Customized Reports Metrolab 4000 Monitor PC Monitor PC Data Management System LIS ASTM Standard serial connection

26. Low Water Consumption 4 mL / test

27. // Current Line - Main Instruments medium labsbig labs / hospitalssmall labs M4000 600 test/hr 400 200 M2300v4 M2300GL M1600DRv3 semiautomatic 10-40 patients/day 240 test/hr 20-60+ patients/day 280 test/hr 60-250+ patients/day 600 test/hr veterinary

28. Clinical Chemistry Analyzers METROLAB 2300 PLUS version 4

29. FULLY AUTOMATIC RANDOM ACCESS CLINICAL ANALYZER End Point Kinetics Immunoturbidimetry Coagulation ISE Barcode: Samples & Reagents Automatic Sample Dilution Mixer on Probe Quality Control Version 4

30. Samples 48 positions for primary tubes, tubes 75 to 100 mm (Ø 13 mm), accepts microtainers. Positive BCR identification of samples Test request through LIS Version 4

31. Reagents 48 positions cooled reagent Bar code for reagents Dual reagent capability 50, 30 and 20 mL containers 24 hours non stop cooling Version 4

32. Cuvette washing system 4-Step washing station Automatic cuvette check 6 mm light path PMMA cuvettes Version 4

33. Multifunction probe Mixer on probe Collision detector Capacitive liquid level detection Effective probe washing Version 4

34. Optical system Double beam 9 interference filters: 340, 405, 505, 550, 590, 650, 700 and 767 nm. Optional filters available (380 and 620 nm ) Version 4

35. Software User friendly Flexible Designed for the clinical analyst Continuous upgrade meeting your requirements Version 4

36. Easy service Open cabinet with only 4 screws Calibration through PC Version 4

37. // Current Line - Main Instruments medium labsbig labs / hospitalssmall labs M4000 600 test/hr 400 200 M2300v4 M2300GL M1600DRv3 semiautomatic 10-40 patients/day 240 test/hr 20-60+ patients/day 280 test/hr 60-250+ patients/day 600 test/hr veterinary

38. Clinical Chemistry Analyzers METROLAB 2300 GL

39. Easy service Calibration through PC No cooling system No washing system No BCR No ISE METROLAB 2300 GL

40. Quick cuvette load Simple and Fast Double tray One tray in use (80 cuvettes) Preloaded tray ready to use METROLAB 2300 GL

41. Clinical Chemistry Analyzers Common Hints

42. Clinical Chemistry Analyzers Common Hints Pipetting principle Semi-disposable plastic cuvettes Low water consumption Probe technology Line style

43. Clinical Chemistry Analyzers Common Hints Proprietary Technology Proprietary software Open or closed customized systems Dedicated after-sales service

44. Clinical Chemistry Analyzers Next Steps… Modular electronics Reduce manufacturing costs Standardization

45. Clinical Chemistry Analyzers Thank you for your attention !!!