1. Introducing automated ELISA - ChemWellTM
Geoffrey Anderson
Unitech Scientific LLC
Lakewood, CA
Visit us:

2. ChemWellTM Wine Autoanalyzer
Manufactured by Awareness Technology
Unitech Scientific - Exclusive Distributor

3. ChemWell uses microwells for all reactions, chemistry and EIA.
First analyzer in the world to combine biochemistry and microplate EIA into one instrument.
ChemWell uses microwells for all reactions, chemistry and EIA.

4. A ChemWell Tour Optical system 2 syringe pumps Wash head
Windows 95, 98 or XP
Wash
and
rinse
bottles
reactions
Reagents
Samples
DI water

5. Designed for labs doing:
ChemWell
Designed for labs doing:
tests per day or
400 EIA per 8 hours
Recommended workload

6. Continuous Sample Loading “Stat” sample priority Low Operating Cost
ChemWell Features & Benefits
Easy to Use
Continuous Sample Loading
“Stat” sample priority
Low Operating Cost
Intuitive, Open Software
Proven, Reliable Technology

7. Calibration Options Cut-off (Semi Qualitative)
Multi-point Polynomial fit
Cubic Spline fit and more
Store Calibration Curves
Wine Controls: Chart Levy Jennings

8. ChemWell runs ELISA as well as Enzymatic reagents
Unitech offers Histamine ELISA kits
Botrytis and Aspergillus ELISA kits
are in development
So let’s take a quick review of the steps and principals of ELISA reagents.

9. Immuno Complex: an Antigen with its matching Antibody
Ag stands for Antigen Ag
Ab stands for Antibody Ab
An antigen and its antibody fit together like 3 dimensional puzzle pieces.

10. Diagram of a simple ELISA with a positive sample (sample has Ag in it)
Mix the sample and diluent together in a microwell that is pre-coated with specific Ab
Incubate for x minutes until the Ag in the sample binds to the Ab on the well walls.
Start with an Add the sample Incubate until
Ab-coated well with some Ag in it complexed Ag Ab Ab
Ab-Ag Ag
Ab-Ag Ab Ab Ab Ab Ab Ab

11. Conjugate is a specific Ab bound to an Enzyme.
Wash out the Add a Incubate until
remaining sample conjugate complexed
Ab-E
Ab-Ag-Ab-E
Ab-Ag
Ab-Ag
Ab-E Ab Ab Ab
Ab-Ag-Ab-E
Ab-Ag
Ab-Ag
Ab-E
Ab-E
The conjugate has a second antibody to the same antigen, allowing you to make
an antigen sandwich (with antibody bread). It also leaves an enzyme hanging off.

12. Washing . . . removes the unbound conjugate, without removing bound antibodies.
Wash out the Add a Substrate + Enzyme
remaining conjugate substrate produces color S
Ab-Ag-Ab-E-S
Ab-Ag-Ab-E
Ab-Ag-Ab-E Ab Ab Ab
Ab-Ag-Ab-E-S
Ab-Ag-Ab-E S
Ab-Ag-Ab-E S S
The more Enzyme available, the more color will develop.
Many ELISA’s use a stopping solution to end the color generating step before
reading.

13. Reliable Results: Enzymatic and ELISA
Precise pipetting
Washes probe inside and out
Liquid sensing
Temperature controlled

14. Easy to Maintain Proven Reliability, over 500 sold Self Diagnosis
Phone/ support by Unitech
Modular Design

15. Economical to Use
Dramatically reduces LABOR
Laboratory
Administrative

16. Warranty 1-year parts & labor Warranty Extended Warranty available
6-Month Preventative Maintenance
Free replacement of warranteed parts
Other repairs if required
Loaner instrument within 72 hours

17. Easy to own Price start at $27,500 Reagent rack cooling option
Add $2000

18. ChemWell Distributed by Unitech Scientific