2. Mrs. Hennings

3. What is blood?  Connective tissue- yes TISSUE!  Cells suspended in plasma matrix

4. Where are blood cells made?  In children, the marrow of most of the bones produces blood. But in adults, only the marrow of certain bones -- the spine, ribs, pelvis, and some others -- continues to make blood. Bone marrow that actively produces blood cells is called red marrow, and bone marrow that no longer produces blood cells is called yellow marrow.

5. What kind of life span?  The different kinds of blood cells have different "life spans" -- red blood cells last about 120 days in the bloodstream; platelets about 10 days; and the various kinds of white blood cells can last anywhere from days to years.

7. Blood Components: "Blood Count" – % of Each Component

8. Blood Components: Cells  Erythrocytes  Red Blood Cells (RBC)  O 2 & CO 2 transport  White Blood Cells (WBC)  Immune defense  Phagocytosis  Platelets: clotting  Liquid part- plasma

9. Plasma  Inorganic salts – dissolved ions called the “blood electrolytes”  Plasma is 90% water  Some ions buffer the blood to maintain a normal pH of 7.35- 7.45  Salts are important in keeping the correct osmotic balance of blood  There are other plasma proteins that play important roles.

10. Plasma proteins are important!  Immunoglobulins= antibodies- fight viruses and other foreign agents that invade  Lipid Escort proteins  Clotting factors- help form plug when bleed  The term “serum” refers to blood after the clotting factors have been removed.

11. Erthyrocytes  Red Blood Cells  Most numerous  Main function is oxygen transport  Structure related to function- AGAIN!  Small disks that are biconcave  Shape increases surface area  Mature RBC do not have NUCLEI! Is this true in all animals?  This leaves more space for hemoglobin  Made in bone marrow  Recycled in spleen

12. The red blood cells here are normal, happy RBC's. They have a zone of central pallor about 1/3 the size of the RBC. The RBC's demonstrate minimal variation in size (anisocytosis) and shape (poikilocytosis). A few small fuzzy blue platelets are seen. In the center of the field are a band neutrophil on the left and a segmented neutrophil on the right.

14. Each hemoglobin can bind between 1-4 molecules of oxygen. If holding 4- then it is said to be fully saturated

15. Iron Metabolism: Key to Hemoglobin O 2 Transport Figure 16-8: Iron metabolism

16. Some Diseases of RBCs and O 2 Transport

17. Remember Sickle Cell? Can you see the Sickle shaped cell? What kinds of problems Does this cause? Why is this disorder Highly prevalent in African Americans?

18. White Blood cells  Major job to fight infection  Leukocytes 5 major kinds- (yes you need to know them all! ) 1. Basophil 2. Neutrophil 3. Eosinophil 4. Lymphocyte- B cells and T cells 5. Monocyte

19. Normal WBC: 4,500 to 10,000 cells/mcL

20. There is a basophil in the center of the field which has a lobed nucleus and numerous coarse, dark blue granules in the cytoplasm. They are infrequent in a normal peripheral blood smear, and their significance is uncertain. A band neutrophil is seen on the left, and a large, activated lymphocyte on the right. This is a basophil.

21. Normal Neutrophil: Electron Micrograph

22. In the center of the field is an eosinophil with a bilobed nucleus and numerous reddish granules in the cytoplasm. Just underneath it is a small lymphocyte. Eosinophils can increase with allergic reactions and with parasitic infestations Eosinophil- bilobed nucleus and red granules

23. A normal mature lymphocyte is seen on the left compared to a segmented PMN on the right. An RBC is seen to be about 2/3 the size of a normal lymphocyte

24. Here is a monocyte. It is slightly larger than a lymphocyte and has a folded nucleus. Monocytes can migrate out of the bloodstream and become tissue macrophages under the influence of cytokines. Note the many small smudgy blue platelets between the RBC's. Monocyte Platelet

25. Blood Components: Platelets Thrombocytes  Coagulate, form plug, prevent blood loss  Formed by fragmentation from megakaryoctyes

26. Overview of Hemostasis: Clot Formation & Vessel Repair

27. Hemostasis: Vasoconstriction & Plug Formation

28. Blood clotting Powerful evolutionary adaptation  emergency repair prevents excessive blood loss Cascade reaction Prothrombin FibrinogenFibrin Thrombin fibrin protein fibers build clot platelets seal the hole chemical emergency signals

29. Stem Cells  Multipotent  Stem Cells  These cells are in the red marrow of bones What is the difference between Multipotent and Totipotent Stem Cells?

30. Hematopoiesis: Blood Cell Formation

32. Out with the old in with the new!  Multipotent- divides one daughter stays stem cell the other becomes specialized  Erythrocytes, leukocytes, and platelets form new cells through stem cell division  Erythrocytes live only 3-4 months  Negative feedback controls erythrocyte production

33. Negative Feedback…  Sensitive to oxygen reaching tissues  Controls Erthyrocyte production  If low in oxygen then kidneys make and secrete a hormone called erythropoietin ( EPO)that tells the stem cells to make more.  If too much oxygen then EPO falls down.  Synthetic EPO-treat anemia  Athletes- inject EPO- blood doping banned

35. What do you think about this being Illegal?