1. Mechanisms of FRC-dependent myeloid cell recruitment.
Mechanisms of FRC-dependent myeloid cell recruitment. Flow cytometry–based enumeration of CD11b+F4/80−Ly6G-Ly6C+ inflammatory monocytes in the omentum (A) and peritoneal cavity (B) in mice lacking MYD88 expression in FRCs or in Cre-negative littermates (Ctrl) at the indicated days after intraperitoneal immunization with S. Typhi OmpC/F. (C) Production of the inflammatory mediators TNF (left) and the chemokine CCL2 (right) by fibroblastic stromal cells isolated from the omentum of Myd88-proficient or Myd88-deficient mice. Data are representative of one of three independent experiments with at least triplicate measurements. nd, nondetected. (D and E) Recruitment of CD11b+ myeloid cells (D) and CD11b+F4/80−Ly6G-Ly6C+ inflammatory monocytes (E) into the omentum of the indicated bone marrow chimeric mice on day 4 after immunization with S. Typhi OmpC/F. (F) Flow cytometric analysis of CD11b+ Ly6C+ CCR2+ monocytes in circulation of the indicated mice under naive conditions with representative dot plots is shown on the left. Data are shown as mean values ± SEM and are pooled from six to nine mice from three independent experiments (A and B) and from six to eight mice per group from two independent experiments (D to F); statistical analysis was performed using Student’s t test (A, B, and F) or one-way ANOVA with Dunnett’s multiple comparison test (C to E) with *P < 0.05, **P < 0.01, and ***P <
Christian Perez-Shibayama et al. Sci. Immunol. 2018;3:eaar4539
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