1. MP cells are generated from naïve cells in the periphery.
MP cells are generated from naïve cells in the periphery. (A) SPF and GF mice have an equal number of MP cells. Splenic CD4+ T lymphocytes from 8-week-old wild-type mice housed in SPF or GF conditions were analyzed for expression of CD44 and CD62L. The representative dot plots indicate the frequency of MP and naïve cells among CD4+ T cells. The bar graphs indicate the number (mean ± SD) of MP and naïve CD4+ T cells from each group (n = 3 to 4). Data are representative of two independent experiments. (B and C) Kinetics of splenic MP and naïve CD4+ T cell development and their proliferative capacity. At the indicated ages, (B) the absolute cell number (mean ± SD) of MP and naïve CD4+ T lymphocytes and (C) the frequency (mean ± SD) of Ki67+ cells among MP and naïve cells were monitored in cohort of wild-type mice [n = 2 to 4 and 2 to 8 per time point in (B) and (C), respectively]. The representative dot plots show the frequency of MP and naïve cells among CD4+ T cells of 1- and 28-day-old mice. Data are pooled from at least two independent experiments. (D) MP CD4+ T cells in Rag2-GFP reporter mice are GFP−. The histogram shows the GFP+ fraction among splenic CD4+ T cells in Rag2-GFP reporter mice, whereas the dot plots and the bar graph indicate the frequency of MP and naïve cells among GFP− and GFP+ CD4+ T cells (n = 5). Data are representative of two independent experiments. (E and F) A normal-sized MP cell population is generated from peripheral CD4+ T cells in the absence of the thymus. (E) The graph shows the frequency (mean ± SD) of CD4+ T cells in the blood from sham-operated and TXD1 mice (n = 3 to 17 per time point) over a 6-month period. (F) Numbers in the representative plots indicate the frequency of MP and naïve cells among peripheral blood CD4+ T lymphocytes on day 141. The bar graphs show the number (mean ± SD) of MP and naïve CD4+ T cells from each group (n = 5 to 6). Data are pooled from at least two independent experiments. *P < 0.05, **P < 0.01, ***P <
Takeshi Kawabe et al. Sci. Immunol. 2017;2:eaam9304
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