1. YU238259 specifically inhibits HDR activity.
YU specifically inhibits HDR activity. A, schematic of DR-GFP and EJ5-GFP cell-based reporter assays for HDR and NHEJ, respectively. B, effect of YU on the frequency of HDR and NHEJ, as assessed by FACS analysis of GFP+ cells. C, relative percentage of cells positive for DNA repair foci at 8 hours after irradiation with 10 Gy IR, following pretreatment with 25 μmol/L YU or vehicle. D, neutral comet assay of DLD-1 or DLD-1 BRCA2-KO cells pretreated with YU and irradiated with 5 Gy IR. DSB levels, as assessed by comet tail moment, were measured at 0 and 24 hours after irradiation. E, cell cycle analysis of DLD-1 and DLD-1 BRCA2-KO cells treated with YU for 6 hours and stained with propidium iodide.
Gregory C. Stachelek et al. Mol Cancer Res 2015;13:
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