Presentation is loading. Please wait.

Presentation is loading. Please wait.

Fig. 6. Bj mutants show stereocilia patterning defects and biliary duct (BD) malformations. Bj mutants show stereocilia patterning defects and biliary.

Published byEva Sundqvist Modified over 4 years ago

Similar presentations

Presentation on theme: "Fig. 6. Bj mutants show stereocilia patterning defects and biliary duct (BD) malformations. Bj mutants show stereocilia patterning defects and biliary."— Presentation transcript:

1 Fig. 6. Bj mutants show stereocilia patterning defects and biliary duct (BD) malformations.
Bj mutants show stereocilia patterning defects and biliary duct (BD) malformations. (A-D) Immunostaining with phalloidin (green) and Vangl2 (red) of the cochlea of near-term wild-type (A,C) and Bj mutant (B,D) embryo. The phalloidin staining delineated the normal chevron-shaped stereocilia in the outer hair cells (OHCs), showing malalignment in the Bj mutant (boxed B) compared to wild-type (boxed A). In addition, Vangl2 staining revealed a subtle misalignment of supporting cells connecting the inner hair cells (IHCs) in the Bj mutant (B), better seen in the enlarged views shown in panels C and D (see arrowheads). (E-J) Shown are bile ducts from a wild-type newborn mouse (E) (n=6) as compared to that of a Bj mutant (F) (n=6), which is noticeably shorter. Quantitative analysis showed a significant decrease in both the length and width of the Bj mutant biliary duct (G). Examination of hematoxylin and eosin stained sections of newborn wild-type (H) (n=4) and Bj mutant (I) biliary ducts suggest a decrease in the mucosal folds of the mutant duct (I) (n=5). This was confirmed with quantitative analysis, which showed a significant decrease in mucosal folds in the mutant duct (J). Error bars show standard deviation. (K-P) Immunostaining with antibodies against β-catenin (red) and E-cadherin (green) showed β-catenin expression is markedly reduced in the Bj mutant biliary duct (N,P), but no change was observed for E-Cadherin (O,P,). Scale bars: 100 µm in A-E); 30 µm (K-P). The P values were calculated with a two-sample Wilcoxon rank-sum test. GB, gallbladder; BD, bile duct. Brian C. Gibbs et al. Biology Open 2016;bio © Published by The Company of Biologists Ltd

Download ppt "Fig. 6. Bj mutants show stereocilia patterning defects and biliary duct (BD) malformations. Bj mutants show stereocilia patterning defects and biliary."

Similar presentations

The neuroepithelium of mid-neurulation mammalian embryos displays planar-polarised F-actin profiles. The neuroepithelium of mid-neurulation mammalian embryos.

The neuroepithelium of mid-neurulation mammalian embryos displays planar-polarised F-actin profiles. The neuroepithelium of mid-neurulation mammalian embryos.
Impaired TrkB signaling in the cerebellum of Pex14ΔC/ΔC BL/ICR mice.

Impaired TrkB signaling in the cerebellum of Pex14ΔC/ΔC BL/ICR mice.
Orientation defects in stereociliary hair bundles with uncoupling from kinocilium and basal body position of hair cells in the organ of Corti of neonatal.

Orientation defects in stereociliary hair bundles with uncoupling from kinocilium and basal body position of hair cells in the organ of Corti of neonatal.
KIN-3 depletion leads to increased levels of centrosomal ZYG-1

KIN-3 depletion leads to increased levels of centrosomal ZYG-1
Defect of cerebellar development and malformation of Purkinje cells in Pex14ΔC/ΔC BL/ICR mice. Defect of cerebellar development and malformation of Purkinje.

Defect of cerebellar development and malformation of Purkinje cells in Pex14ΔC/ΔC BL/ICR mice. Defect of cerebellar development and malformation of Purkinje.
Fig. 6. crb_C mutant photoreceptor cells exhibit normal morphology.

Fig. 6. crb_C mutant photoreceptor cells exhibit normal morphology.
Fig. 5. Prip silencing enhances the co-localization of GABARAP with insulin vesicles and β-tubulin.Co-localization of GABARAP (green) with insulin (red)

Fig. 5. Prip silencing enhances the co-localization of GABARAP with insulin vesicles and β-tubulin.Co-localization of GABARAP (green) with insulin (red)
Fig. 1. Overview of the nervous system of the adult S. roscoffensis.

Fig. 1. Overview of the nervous system of the adult S. roscoffensis.
Fig. 4. Clinically defined SMN mutants show alterations in the association with telomerase complex proteins. Clinically defined SMN mutants show alterations.

Fig. 4. Clinically defined SMN mutants show alterations in the association with telomerase complex proteins. Clinically defined SMN mutants show alterations.
Fig. 3. Hts and Dlg are in a complex at the postsynaptic membrane of larval NMJs.(A–B″) PLA with HtsM and Dlg antibodies (green) performed on third instar.

Fig. 3. Hts and Dlg are in a complex at the postsynaptic membrane of larval NMJs.(A–B″) PLA with HtsM and Dlg antibodies (green) performed on third instar.
Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.

Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.
Fig. 4. E-cadherin expression level affects monomer dynamics.

Fig. 4. E-cadherin expression level affects monomer dynamics.
Fig. 4. smc3 regulates the expression of cx43 in regenerating fins.

Fig. 4. smc3 regulates the expression of cx43 in regenerating fins.
Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.
Fig. 5. Differentiated cells sort to the outer layer, regardless of E- or N-cadherin status.Wildtype and mutant ES cells were distinguished by GFP expression.

Fig. 5. Differentiated cells sort to the outer layer, regardless of E- or N-cadherin status.Wildtype and mutant ES cells were distinguished by GFP expression.
Fig. 2. Proportion of motile objects and track length

Fig. 2. Proportion of motile objects and track length
Fig. 1. Bj mutant exhibits outflow tract malalignment defects.

Fig. 1. Bj mutant exhibits outflow tract malalignment defects.
Fig. 3. Knockdown of cited3 results in increased cell death but it does not affect proliferation.Embryos that are injected with the control MO (A–C), cited3.

Fig. 3. Knockdown of cited3 results in increased cell death but it does not affect proliferation.Embryos that are injected with the control MO (A–C), cited3.
Fig. 6. Cross-section of the stomach wall and spiral intestine of the embryo, stained with PAS. (A) Surface of the stomach wall (SW) and ingested material.

Fig. 6. Cross-section of the stomach wall and spiral intestine of the embryo, stained with PAS. (A) Surface of the stomach wall (SW) and ingested material.
Fig. 1. Prestin in mouse outer hair cells is localized along the lateral wall of the cell along with β-catenin and Na/K ATPase.Shown are cartoons of the.

Fig. 1. Prestin in mouse outer hair cells is localized along the lateral wall of the cell along with β-catenin and Na/K ATPase.Shown are cartoons of the.

Similar presentations

Ads by Google