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The neuroepithelium of mid-neurulation mammalian embryos displays planar-polarised F-actin profiles. The neuroepithelium of mid-neurulation mammalian embryos.

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Presentation on theme: "The neuroepithelium of mid-neurulation mammalian embryos displays planar-polarised F-actin profiles. The neuroepithelium of mid-neurulation mammalian embryos."— Presentation transcript:

1 The neuroepithelium of mid-neurulation mammalian embryos displays planar-polarised F-actin profiles.
The neuroepithelium of mid-neurulation mammalian embryos displays planar-polarised F-actin profiles. (A) Whole-mount immunofluorescence image of an E9.5 mouse embryo stained for Vangl2, showing expression in the posterior neuropore (PNP) as well as surrounding surface ectoderm (SE). Vangl2 colocalisation with the SE marker E-cadherin (E-Cad) is shown in an optical slice (located within the white line box next to the zippering point, which is indicated by an asterisk). (B) Representative phalloidin-stained E9.5 mouse PNP showing the presence of a supracellular F-actin cable along the neural folds (arrows) and F-actin enrichment on the apical surface of the PNP (white line box, enlarged in C-E). (C) Fire LUT of the boxed area in B, highlighting F-actin enrichment in red. (D) The same image shown binarised to illustrate ML profiles (cyan arrows) of enriched borders. (E) Enlarged region from the boxed area in C, showing F-actin enrichment of ML-oriented cell borders (cyan arrows) relative to RC-oriented borders between them, forming a ladder-like pattern. Scale bars: 100 µm. Gabriel L. Galea et al. Dis. Model. Mech. 2018;11:dmm032219 © Published by The Company of Biologists Ltd

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