1. Fig. 2 AcPGP induces IL-8 and G-CSF release from human bronchial epithelial cells.
AcPGP induces IL-8 and G-CSF release from human bronchial epithelial cells. (A and C) Normal human bronchial epithelial cells were cultured at air-liquid interface. Respective wells were treated with media or media supplemented with AcPGP (10 μg/ml). Apical supernatants were collected on day 1 for measurement of IL-8 (A) and G-CSF (C) by enzyme-linked immunosorbent assay (ELISA). (B and D) Undifferentiated normal human bronchial epithelial cells were cultured in media or media supplemented with AcPGP (10 ng/ml) or PGP (100 ng/ml) for 72 hours. In some groups, AcPGP-treated cells were preincubated with either anti-CXCR1/2 antibodies or IgG2a isotype control antibody. Supernatant was collected for the measurement of IL-8 (B) and G-CSF (D) by ELISA. (A) and (C) present combined data from two independent experiments with two wells per group in each experiment. (B) and (D) represent data combined from two independent experiments with five wells per group in each experiment. Results are depicted as means ± SEM. *P < 0.05, **P < 0.01, and ****P < , using Mann-Whitney statistical test (A and C) or ANOVA with Bonferroni correction (B and D).
Dhiren F. Patel et al., Sci Transl Med 2019;11:eaaw0462
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