1. Crotonate prevents downregulation of kidney PGC-1α and SIRT3 as well as causing CCL2 upregulation in experimental AKI. AKI was induced by a folic acid overdose in mice pretreated with or without 12 mmol/kg body weight crotonate.
Crotonate prevents downregulation of kidney PGC-1α and SIRT3 as well as causing CCL2 upregulation in experimental AKI. AKI was induced by a folic acid overdose in mice pretreated with or without 12 mmol/kg body weight crotonate. All mice were killed at 72 h. (A) Crotonate prevented the increase in serum BUN and creatinine levels and in Kim-1 mRNA expression observed in AKI. Mean±s.e.m. of five mice per group. *P<0.05 vs vehicle control, #P<0.05 vs vehicle AKI (non-parametric Mann–Whitney U-test). (B-D) The kidney PGC-1α mRNA decrease (B), SIRT3 mRNA decrease (C), and CCL2 mRNA increase (D) were prevented by pretreatment with crotonate. Mean±s.e.m. of five animals per group. *P<0.05 vs vehicle control, #P<0.05 vs vehicle AKI (non-parametric Mann–Whitney U-test). (E,F) Kidney PGC-1α (E) and SIRT3 (F) protein levels decreased in AKI and this was prevented by pretreatment with crotonate. Western blot of whole kidney protein. Mean±s.e.m. of five animals per group. *P<0.05 vs vehicle control, #P<0.05 vs vehicle AKI (non-parametric Mann–Whitney U-test).
Olga Ruiz-Andres et al. Dis. Model. Mech. 2016;9:
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