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Fig. 1. Muscle-specific PITX1 over-expression in the Pitx1 transgenic mice.(A) Detection of Pitx1 mRNA expression in muscles of the Pitx1 transgenic mice.

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Presentation on theme: "Fig. 1. Muscle-specific PITX1 over-expression in the Pitx1 transgenic mice.(A) Detection of Pitx1 mRNA expression in muscles of the Pitx1 transgenic mice."— Presentation transcript:

1 Fig. 1. Muscle-specific PITX1 over-expression in the Pitx1 transgenic mice.(A) Detection of Pitx1 mRNA expression in muscles of the Pitx1 transgenic mice after PITX1 over-expression was induced for five weeks. Muscle-specific PITX1 over-expression in the Pitx1 transgenic mice.(A) Detection of Pitx1 mRNA expression in muscles of the Pitx1 transgenic mice after PITX1 over-expression was induced for five weeks. Pitx1 transcripts were detected in the two muscles, gastrocnemius and quadricep, examined (lanes 1 and 2), but not in the brain, heart, lung, liver, kidney or testis (lanes 3–8). Lane 9 is a negative control using RNA of quadricep without reverse transcriptase. (B) The expression levels of PITX1 protein in the triceps and quadriceps of the Pitx1 transgenic mice and control mice (mCK-tTA) after PITX1 over-expression was induced for five weeks. “*” indicates p<0.05. (C) Immunoblotting images of PITX1 and GAPDH. DT, Pitx1 transgenic mice; C, control mice. B and C showed PITX1 protein was 3.3 and 2.9 fold higher in the triceps and quadriceps than in those of the control mice, respectively. Sachchida N. Pandey et al. Biology Open 2012;bio © Published by The Company of Biologists Ltd

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