1. Blood Tfr cells are lymphoid tissue–derived Tfr precursors.
Blood Tfr cells are lymphoid tissue–derived Tfr precursors. (A) CD45RO+CCR7− effector memory, CD45RO+CCR7+ central memory, and CD45RO−CCR7+ naïve subsets of Tfr cells and CXCR5− Treg cells in children blood (top) and tissues (bottom). Representative plots (left) and pooled data (right) (n = 6; Student’s t test) are shown. Tfh cells are represented in blue, CXCR5− Treg cells in black, and Tfr cells in red. CXCR5+ subsets in tonsils were defined as CXCR5+ICOS+ cells (fig. S2B). (B) Blood Tfh and Tfr cells from X-linked agammaglobulinemia (BTK-deficient) patients compared with sex- and age-matched HDs. Representative plots (left) and pooled data (right) (n = 5; Student’s t test) are shown. (C) Model of CXCR5+ Tfh and Treg cell generation and recirculation in humans upon antigen stimulation. Tfh cells are shown in red, and Tfr cells in blue. DC, dendritic cell. (D) Frequency of peripheral blood Tfr cells on the day of influenza vaccination (d0) and 7 days later in healthy volunteers. Schematic representation and representative plots (left) and pooled data (right) (n = 32; Student’s t test) are shown. Bars represent SEM.
Valter R. Fonseca et al. Sci. Immunol. 2017;2:eaan1487
Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works