1. FIP200 deficiency alters mitochondria activation and ROS production in T cells.
FIP200 deficiency alters mitochondria activation and ROS production in T cells. (A and B) The mitochondria contents (A) and ROS production (B) were analyzed by FACS in naïve T cell subsets in WT and Fip200−/− mice (n = 4 to 5, means ± SEM). *P < 0.05 (Mann-Whitney U tests). MFI, mean fluorescence intensity. (C) Effects of NAC on WT and Fip200−/− T cell apoptosis in vitro. T cell subsets were treated with 0.1 mM NAC for 12 hours. Numbers on the dot plots represent the percentage of Annexin V+ T cells (n = 3). (D and E) Effects of NAC on WT and Fip200−/− T cell apoptosis in vivo. Mice were treated daily with NAC for 4 weeks. (D) Apoptosis was determined by Annexin V staining in activated T cells. (E) The absolute numbers of naïve CD4+ and CD8+ T cells were quantified in lymph nodes in WT and Fip200−/− mice without or with NAC treatment (n = 4 to 5, means ± SEM). *P < 0.05 (Mann-Whitney U tests) between KO mice without and with NAC treatment. (F and G) Effects of metformin on WT and Fip200−/− naïve T cell ROS and apoptosis in vivo. Mice were treated daily with metformin for 2 weeks. (F) The ROS production was analyzed by FACS in naïve T cell subsets in WT and Fip200−/− mice. (G) The percentage of Annexin V+ T cells in lymph node and spleen from WT and Fip200−/− mice (n = 3, means ± SEM). *P < 0.05 (Mann-Whitney U tests) between KO mice without and with metformin treatment.
Houjun Xia et al. Sci. Immunol. 2017;2:eaan4631
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