1. Definition of the cellular source of IL-22.
Definition of the cellular source of IL-22. (A) Weight and colonoscopic analysis of [Cx3cr1cre:Il10rafl/fl > WT], [Cx3cr1gfp/+ > WT], or [Cx3cr1cre:Il10rafl/fl:Il-22−/− > WT] BM chimeras. Colonoscopy was performed 6 weeks after transplant. n.s., non significant, P > 0.05; *P ≤ Data were collected from two independent experiments, n ≥ 3 in each. (B) qRT-PCR analysis of Il22, Cxcl1, and Cxcl5 expression in whole-tissue extracts of colons of indicated BM chimeric mice. Data were collected from two independent experiments, n = 3 to 5 in each group. (C) qRT-PCR analysis of Il22 expression in whole-tissue extracts of colons of indicated BM chimeric mice. Data are from one of two representative experiments. Weight (left) and colonoscopic (right) analysis of [Cx3cr1cre:Il10rafl/fl:Il22−/− > WT] and [Cx3cr1cre:Il10rafl/fl:Il22−/− > Il22−/−] BM chimeras. Colonoscopy was performed 7 weeks after transplant. Weight data are from one of two representative experiments, n = 3 to 4 in each group; colonoscopic data are collected from two experiments. n = 3 to 4 in each group. (D) Flow cytometry analysis of the lamina propria of [Cx3cr1cre:Il10rafl/fl > Rorgtgfp] and [Il10rafl/fl > Rorgtgfp] BM chimeras. Tcrb, T cell receptor beta chain. Data are representative of three experiments, n = 3 to 5 in each group. (E) qRT-PCR analysis of Il22 expression by sorted TH17 cells from indicated BM chimeras. Data were collected from three independent experiments, n = 3 to 5 in each group. (F) Weight and colonoscopic analysis of [Cx3cr1cre:Il10rafl/fl > WT] or [Cx3cr1cre:Il10rafl/fl > Il-22−/−] BM chimeras. Colonoscopy was performed 6 weeks after transplant. Data were collected from two independent experiments, n ≥ 3 in each group. (G) qRT-PCR analysis of Il22 and Cxcl5 expression in whole-tissue extracts of colons of indicated BM chimeric mice. Data were collected from two independent experiments, n = 3 to 5 in each group.
Biana Bernshtein et al. Sci. Immunol. 2019;4:eaau6571
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