1. Differential expression of TRM markers by donor- and recipient-derived T cells with time.
Differential expression of TRM markers by donor- and recipient-derived T cells with time. (A) Expression of CD69 and CD103 by CD4+ (top two rows) and CD8+ T cells (third and fourth rows) of donor or recipient origin as indicated over time after transplantation for one representative patient, P5. (B) Graphs show mean frequency (±SEM) of CD69 expression (left) or CD69/CD103 coexpression (right) by CD4+ TEM (top) or CD8+ TEM (bottom) cells in patient BAL at indicated times after transplantation, compiled from 20 patients. T cell origin designated as donor (blue) and recipient (red) based on HLA disparities (see Fig. 1); gray shaded rectangles denote 1 SD around the average frequency of CD69+ (left) and CD69+CD103+ (right) expression by control BAL T cells compiled from Fig. 2D. Expression of (C) CD49a, (D) PD-1, and (E) CD101 by BAL, airways, and parenchyma obtained from control lungs shown as representative flow cytometry plots and mean frequencies (±SEM), compiled from 14 lungs. *P = 0.02 (CD101); *P < 0.05 and **P = (CD49a); ns (PD-1). (F) CD49a expression by donor (black) and recipient (red) CD4+ TEM (top) and CD8+ TEM (bottom) cells in patient BAL samples shown as representative flow cytometry plots (left) and in graphs showing paired frequencies from individual patient BAL samples (n = 6) at >1 month after transplantation. ***P = and *P = (G) PD-1 expression by donor (black) and recipient (red) CD4+ TEM (top) and CD8+ TEM (bottom) cells in patient BAL shown as representative flow cytometry plots (left) and graphs showing paired frequencies in patient BAL samples (n = 14) >6 months after transplant. ***P = (H) CD101 expression by donor (black) and recipient (red) CD4+ TEM (top) and CD8+ TEM (bottom) cells in representative flow cytometry plots (left) and paired frequencies in patient BAL samples (n = 15) at >1 month after transplantation. *P = 0.02.
Mark E. Snyder et al. Sci. Immunol. 2019;4:eaav5581
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