1. Effects of KY‐02327 on OVX‐induced osteoporosis model mice
Effects of KY‐02327 on OVX‐induced osteoporosis model mice A–HVehicle or 20 mg of KY‐02327 per kg of animal body weight (mpk) was orally administered or 0.06 mpk of the N‐terminal fragment of PTH (amino acids 1‐34) was subcutaneously injected into the Sham‐operated (Sham) or ovariectomized (OVX) mice on 5 sequential days per week for 4 weeks (n = 4). After calcein injection, the prepared femoral sections of the OVX mice were examined under fluorescence microscope to visualize integrated calcein (A, green, arrows). Nuclei were counterstained with DAPI (A, blue). Scale bars, 100 μm. Osteoblast numbers (B) and calcein double‐labeled surfaces (C) were measured in the femoral sections. The mouse femurs were analyzed using micro‐CT, and BMD (D) and BV/TV (E) were calculated from the micro‐CT data. The three‐dimensional images of femoral trabecular bone were reconstructed (F), and trabecular number (G) and trabecular separation (H) were calculated. Data Information: The data are the mean ± s.d. (error bars). Significance was assessed using Kruskal–Wallis test.
Hyun‐Yi Kim et al. EMBO Mol Med. 2016;8:
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