1. Neuro‐inflammatory cytokines, brain microglial activation, and working memory deficits are normalized using LV.IDS.ApoEII, but not LV.IDS in MPS II mice
Neuro‐inflammatory cytokines, brain microglial activation, and working memory deficits are normalized using LV.IDS.ApoEII, but not LV.IDS in MPS II mice A–DCytokine bead arrays measuring (A) MIP‐1α, (B) IL‐1α, (C) RANTES, and (D) MCP‐1 levels in whole‐brain lysate of 8‐month‐old mice using flow cytometry (n = 6 mice/group).ERepresentative images of 30‐μm sections of the motor cortex (M2) and striatum stained with isolectin B4 (ILB4) to identify activated microglia, 40×. Scale bar: 50 μm.F, GFour 30‐μm sections per mouse of the cortex (F) and striatum (G) were counted for the number of ILB4‐positive cells (0.26 to −1.94 mm from bregma), n = 3 mice/group.HSchematic of the Y‐maze to measure working memory.IPercentage alternation as an indicator of working memory was measured in 10‐min trials in the Y‐maze at 8 months of age. n = 12–16 mice per group.Data information: Data are mean ± SEM, one‐way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001, ****P <  vs. MPS II; other comparisons are indicated by brackets.
Hélène FE Gleitz et al. EMBO Mol Med. 2018;10:e8730
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