1. Reduced scar formation in the injured GM of CCR2−/− mice
Reduced scar formation in the injured GM of CCR2−/− mice A, BRepresentative images depict the GFAP immunoreactive area at 28 dpi in WT (A) and CCR2−/− mice (B).C, DTriple immunolabeling with NeuN, CD45, and GFAP in WT (C) and CCR2−/− mice (D) with higher magnifications to depict the NeuN‐free areas in the injured GM at 28 dpi (C′, D′) that are colonized by CD45+ macrophages (C′–D″).E–GHistograms in (E–G) depict percentages of the area covered by immunoreactivity to GFAP (Unpaired t‐test, *P = 0.027, n = 3) (E), NeuN (Unpaired t‐test, *P = 0.038, n = 3) CD45 (Unpaired t‐test, *P = 0.011, n = 3) (F) and CD45 (Unpaired t‐test, *P = 0.011, n = 3) NeuN (Unpaired t‐test, *P = 0.038, n = 3) (G) normalized to the acquired area for each slice.H–KRepresentative images of NeuN staining of the uninjured contralateral hemisphere (H, I) and ipsilateral lesion site (J, K), displaying the regions of interest (ROIs) selected for quantification of the number of NeuN+ cells.L–QHigher magnifications of the ROIs.RHistogram depicting the number of NeuN+ cells in ROI of the uninjured contralateral hemisphere, as well as ROI1 and ROI2 at the injury site. Statistical significance was examined with one‐way ANOVA (P < ) with Tukey's post hoc test, *P < 0.05, ns P > 0.05, n = 3.Data information: All data (individual data points, i.e., animals, are depicted as dots or squares) are represented as mean ± SEM. Scale bars: 100 μm. The dashed lines indicate the site of injury.
Jesica Frik et al. EMBO Rep. 2018;embr
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