1. Phylogenetics of Cyprinodontiformes
J.Ryce Martin

2. Introduction
Pre 1981, Cyprinodontiformes (order of killifishes) classified by viparity
Now combination of morphological and molecular data are used to calculate relatedness
I used sequence alignment of the cytochrome b gene to construct a strict consensus parsimony tree, bootstrap values for the parsimony tree, and a distance neighbor-joining tree

3. 50% majority rule consensus of 9 parsimony trees
Fundulidae
Rivulidae
Aplocheilidae
Poecilidae
50% majority rule consensus of 9 parsimony trees
Fundulids are separated by two separate families
Lucania may be part of fundulidae but also has similar sequences to Rivulids and Aplocheilids
could be evidence to polyphyletic origins
all individuals in three families are closely related almost creating own clade
Can see why everything was grouped in Cyprinodontidae
Anablepids are seeming less related to all other taxa
Goodeidae
Profundulidae
Cyprinodontidae
Anablepidae

4. My taxa of fundulidae at top is dividing costa’s similar clade
Rivulidae
Aplocheilidae
Fundulidae
Profundulidae
Goodeidae
Poeciliidae
Valenciidae
Anablepidae
Profundulus
Cyprinodontidae
Beloniformes
My parsimony tree
Costa 1998
My taxa of fundulidae at top is dividing costa’s similar clade
Goodeidae and profundulidae work good, they should be off of fundulidae
If goodeidae and profundulidae were moved to to lower fundulidae, then tree may look a little better

5. Rivulidae
Aplocheilidae
Fundulidae
Profundulidae
Poeciliidae
Valenciidae
Anablepidae
Goodeidae
Profundulus
Cyprinodontidae
Beloniformes
My parsimony tree
Parenti 1981

6. Ran bootstrap in same order of text file
100 92
100
100 98 93 97 83 96 87
100 72 99
100 92
100 80 87
Ran bootstrap in same order of text file 69 74
100
100

7. Neighbor-joining of distance between taxa
taxa with less distance join together
taxa with next less distance join on next node
Neotropical fish are mixed up

8. Conclusions Parsimony and Neighbor-Joining did not match up well
Could be due to different methods used
Bootstrap values appeared pretty good
Many branches collapsed creating polytomies
Hard polytomy
Family tree doesn’t match up well to literature
I used cyt b they used morphology and Trysine kinase X-src gene