1. Postgestational effects of macrophage migration inhibitory factor on embryonic implantation in mice 
Patrick Kibangou Bondza, Ph.D., Christine N. Metz, Ph.D., Ali Akoum, Ph.D. 
Fertility and Sterility 
Volume 90, Issue 4, Pages (October 2008)
DOI: /j.fertnstert
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Effect of macrophage migration inhibitory factor (MIF) on the mean embryo weight at day 8 of pregnancy. Embryos flushed from control and MIF-treated mice were gently weighed. Data represent the mean weight ± SEM of embryos in all litters from control (n = 12) and MIF-treated mice (0.15 μg/mL, n = 10 ; 1.5 μg/mL, n = 11, and 10 μg/mL, n = 6). A one-way ANOVA and Dunnett test for post-hoc multiple comparisons were used to assess differences. No significant differences were statistically found.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Scattergram representation of the effect of macrophage migration inhibitory factor (MIF) treatment on αv (A), β3 (B), and Vegf (C) mRNA expression by quantitative real-time PCR. For each factor, the ratio of mRNA level to Gapdh mRNA was determined. Results were compared between control groups (n = 12) and MIF-treated groups: 0.15 μg/mL (n = 10), 1.5 μg/mL (n = 11), and 10 μg/mL (n = 6). The horizontal lines represent the mean for each set of data (∗P<.05 ; ∗∗P<.01, significantly different from control group).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Immunohistochemical detection of αv (A, B) and β3 (D, E) integrin subunits, and VEGF (G, H) in the mouse endometrium. Note the strong positive brownish immunostaining of these factors in endometrial glands and surface epithelium from a mouse treated with 10 μg/mL macrophage migration inhibitory factor (A, D, G) in comparison with endometrium from a mouse that received a saline solution as control (B, E, H), whereas stromal cells remain weakly or not stained in both groups. The specificity of immunostaining was confirmed by the absence of any staining when primary antibody were replaced with an equal concentration of adequate nonimmune rabbit IgGs (C, F, I). Original magnification, x400.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Scattergram representation of immunostaining for αv (A), β3 (B), and VEGF (C) in control and macrophage migration inhibitory factor (MIF)-treated mice. The intensity and distribution of staining were determined only in endometrial epithelial glands and surface epithelium (HSCORE) due to the absent or weak staining in stromal cells. One-way ANOVA and the Dunnett test for post-hoc multiple comparisons were used to assess significant differences. The horizontal lines represent the mean for each set of data (∗P<.05; ∗∗P<.01, significantly different from control group).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

6. Figure 5
Immunohistochemical staining of von Willebrand factor (vWF) in the endometrium of a control mouse (A) versus 10 μg/mL macrophage migration inhibitory factor -treated mouse (B). The positive brown immunostaining of the vessels was observed with rabbit anti-vWF antibody. No immunostaining was observed when primary antibody was replaced with an equal concentration of nonimmune rabbit IgGs (C) or when anti-vWF antibody was incubated with recombinant vWF (D). v = vessels; s = stroma; g = gland. Original magnification, x400.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

7. Figure 6
Correlations between VEGF HSCORE and endometrial microvessels density in controls and macrophage migration inhibitory factor (MIF)-treated mice. A Pearson r correlation coefficient was used in each case, yielding to a positive and significant correlation in mice treated with 10 μg/mL MIF (r = , P=.0207). vWF = von Willebrand factor.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions