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Amir Hassan Zarnani, D. M. T. , Ph. D. , Mehdi Shahbazi, M. S

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Presentation on theme: "Amir Hassan Zarnani, D. M. T. , Ph. D. , Mehdi Shahbazi, M. S"— Presentation transcript:

1 Vitamin D3 receptor is expressed in the endometrium of cycling mice throughout the estrous cycle 
Amir Hassan Zarnani, D.M.T., Ph.D., Mehdi Shahbazi, M.S., Alireza Salek-Moghaddam, Ph.D., Mehri Zareie, Ph.D., Maryam Tavakoli, M.D., Jemileh Ghasemi, B.S., Simin Rezania, M.S., Ali Moravej, M.S., Ebrahim Torkabadi, M.S., Hodjattallah Rabbani, Ph.D., Mahmood Jeddi-Tehrani, Ph.D.  Fertility and Sterility  Volume 93, Issue 8, Pages (May 2010) DOI: /j.fertnstert Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Expression of vitamin D3 receptor (VDR)-specific transcript in the endometrium of cycling mice at different stages of the estrous cycle. After extraction of RNA from endometrium of Balb/c mice at different stages of estrous cycle and cDNA synthesis, RT-PCR was run using specific primer for VDR, and GAPDH amplification was used as the control. (A) Proestrus. (B) Estrus. (C) Metestrus. (D) Diestrus. (E) Negative control with no added cDNA. (F) Mouse kidney as positive tissue control. (G) DNA molecular weight marker. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Immunohistochemical analysis of vitamin D3 receptor (VDR) expression in the endometrium of cycling mice at (A) proestrus, (B) estrus, (C) metestrus, and (D) diestrus. Luminal and glandular epithelial cells were the prominent cell types that expressed VDR in all phases (A–D, black arrows). Immunoreactivity was highest in the estrus phase. There were also scattered positive cells in the endometrium stroma. (E) Negative control. (F) Mouse intestine as positive tissue control. (G) Negative reagent control of mouse intestine. Magnification: ×400. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

4 Figure 3 Immunohistochemical colocalization of vitamin D3 receptor (VDR) in mouse endometrial dendritic cells and macrophages. (A, C) Mouse endometrium immunostained with anti-VDR antibody; (B) immediate serial section immunostained with dendritic cell–specific marker CD11c; or (D) macrophage marker F4/80. Both endometrial dendritic cells and macrophages strongly express VDR (A, C, black arrows). Magnification: ×400. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

5 Figure 4 Immunohistochemical detection of vitamin D3 receptor (VDR) in the fallopian tubes of cycling mice at (A) proestrus, (B) estrus, (C) metestrus, and (D) diestrus. At all stages, epithelial cells were the prominent cell types that expressed VDR (A–D, black arrows). (E) Negative reagent control. Magnification: ×400. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

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