1. Expression and function of fatty acid–binding protein 4 during mouse decidualization 
Zhen Tian, Ph.D., Zhen-Ao Zhao, Ph.D., Xiao-Huan Liang, Ph.D., Xiu-Hong Zhang, Ph.D., Ai-Guo Sha, B.S., Zhi-Rong Zhang, B.S., Yong-Sheng Yu, Ph.D., Zeng-Ming Yang, Ph.D. 
Fertility and Sterility 
Volume 95, Issue 8, Pages e5 (June 2011)
DOI: /j.fertnstert
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Expression and function of Fabp4 in decidua, deciduoma, and stromal cells under in vitro decidualization. (A) Confirmation of Fabp4 expression by real-time RT-PCR. Fabp4 mRNA was significantly up-regulated in day-8 decidua and deciduoma compared with control (uninjected uterine horn). One-way analysis of variance was used. ∗P<.05 was considered statistically significant. (B) Confirmation of Fabp4 expression by Western blot. Western blot showing the high level of FABP4 in day-8 decidua and deciduoma compared with control. (C) Fabp4 expression in mouse uterus during early pregnancy detected by in situ hybridization. (D) FABP4 protein during early pregnancy is shown by immunostaining. Asterisk (∗) indicates embryo. Bar = 60 μm. (E) Effects of Fabp4 knockdown on Dtprp mRNA expression. After transfection with control siRNA or Fabp4 siRNA, stromal cells from 4-week-old mice were induced for in vitro decidualization for 48 h. (F) Effects of Fabp4 overexpression on Dtprp expression. After transfection with control plasmid (empty vector) or Fabp4 overexpression plasmid, stromal cells from 4-week-old mice were induced for in vitro decidualization for 48 h. (G) Effects of Fabp4 inhibitor on Dtprp expression. Dtprp expression was significantly inhibited by FABP4 inhibitor (20 μM) compared with control in day-4 stromal cells under in vitro decidualization for 48 h. ∗P<.05, Student’s t test. (H) FABP4 mRNA level in human endometrium from LH+2 and LH+7. ∗P<.05, Student’s t test. D1-8 = day 1–8; D5(I) = day-5 implantation site; D5(NI) = day 5 interimplantation site; E + P = 17β-E2 plus P.
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

3. Supplemental Figure 1
Fabp4 expression under in vitro decidualization of uterine stromal cells. Dtprp (A) and Fabp4 (B) mRNA expression in uterine stromal cells from 4-week-old mice under in vitro decidualization for 5 and 8 days, respectively. Beta-actin was used as an internal reference gene for normalization. Student’s t test was used. P<.05 was considered statistically significant. (C) Western blot of FABP4 protein in uterine stromal cells from 4-week-old mice under in vitro decidualization for 5 and 8 days, respectively. Dtprp mRNA (D) and FABP4 protein (E) expression in uterine stromal cells from day-4 mice under in vitro decidualization for 48 hours. rPL7 or glyceraldehyde 3-phosphate dehydrogenase was used as an internal reference gene for normalization. Student’s t test was used. P<.05 was considered statistically significant.
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

4. Supplemental Figure 2
Fabp4 expression after transfection of siRNA against Fabp4 or Fapb4 overexpression plasmid. (A) Effect of Fabp4 knockdown on Fabp4 mRNA expression. (B) Effects of Fabp4 overexpression on Fabp4 mRNA expression.
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

5. Supplemental Figure 3
Fabp4 expression under human in vitro decidualization. The expression of IGFBP1 (A), PRL (B), and FABP4 (C) in human endometrial stromal cells under in vitro decidualization for 6 and 9 days was determined by real-time RT-PCR. Glyceraldehyde 3-phosphate dehydrogenase was used as an internal reference gene for normalization. (D) FABP4 mRNA level in human endometrium from LH+2 and LH+7. RPL7 was used as an internal reference gene for normalization. Student’s t test was used. P<.05 was considered statistically significant.
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions