Download presentation
Presentation is loading. Please wait.
1
Intracellular Transport Pathways
2
Three major coats function in the secretory pathway
3
Intracellular Transport Pathways
AP2 AP1, 3
4
Composition of the COPII coat
5
Structure of Sec13/31 cage Stagg et al. Nature 2006
6
COPI coat assembly
7
COPI-coated vesicles
8
Clathrin-coated vesicles
9
Clathrin-coated vesicles
10
Clathrin-coated vesicles
11
Endocytosis requires dynamin GTPase
12
Dynamin forms a neck
13
- Priming: Recruitment of GTP-binding protein GEF localization! - Coat recruitment - Cargo recruitment interactions with coat components fusion machinery components
14
SNARE Hypothesis v- and t-SNAREs are part of the fusion machinery
v- and t-SNAREs provide compartment specificity
16
“SNAREpin model
17
HA-induced membrane fusion
18
SNARE-mediated fusion
19
SNARE-mediated fusion
20
v/t SNARE fusion cycle
21
v/t SNAREs are compartment-specific
From McNew et al. (2000)
22
v/t SNAREs are compartment-specific
From Parlati et al. (2000)
23
compartment-specific
v/t SNAREs are compartment-specific From Parlati et al. (2000)
24
v/t SNAREs are compartment-specific
Parlati et al. (2000)
25
Flipped SNAREs can fuse cells
Hu et al., Science 2003
Similar presentations
© 2024 SlidePlayer.com Inc.
All rights reserved.