1. Lipids-2015
COMPARISON OF THE LIPID CONTENT AND FUMONISINS CONCENTRATION IN MAIZE GENETIC DIVERSITY
Dr. Silvia Denise Peña Betancourt
Toxicology Laboratory
University Autonomous Metropolitan

2. Content Mexican maize diversity Mexican diet
Overweight, obesity and diabetes
Comparation of lipids and fumonisins content
Conclusions

3. Maize In Mexico there are more than 59 races and 2000 varieties
Lipids-2015 Conference
Maize
In Mexico there are more than 59 races and varieties
Native corn

4. Lipids-2015 Conference
The native seed is planting in 50% of national land into small farms with manual agronomic practices depending of rain as water resource

6. We have too new varieties of maize from genetic manipulation
Lipids-2015 Conference
We have too new varieties of maize from genetic manipulation
These varieties expresse recombinant proteins or Crystal proteins
Bt maize
Sequencing of maize genome

7. There are some hybrid maize can be resistant to fungus
Lipids-2015 Conference
There are some hybrid maize can be resistant to fungus
Fusarium it is a commun fungus in agricultural maize soils
Fusarium cause an great loss annual field crop

8. Lipids-2015 Conference
Fusarium verticillioides synthesizing Fumonisins can be harmfull for human

9. The regulation by corn flour is 2-4 ppm
Lipids-2015 Conference
Environmental Protection Agency added Fumonisin B1 to the list of causing cancer substances in California State USA
The regulation by corn flour is 2-4 ppm
FDA, USA established an IDA in 2 ppb

10. The maize we eat came from a great genetic diversity
Lipids-2015 Conference
The maize we eat came from a great genetic diversity
The consume of corn as Tortillas (flat pancake) is 33 g/day by adult person almost 50% of Energy intake needed
Tortillas are rich in starch, saturated, mono and poliunsaturated lipids
121

11. According to National Health and Nutrition in 2012
Lipids-2015 Conference
According to National Health and Nutrition in 2012

12. 6% of Mexican population has Diabetes Type 2 represented 7.32 millions
Lipids-2015 Conference
6% of Mexican population has Diabetes Type 2 represented 7.32 millions
Of diabetes is caused by obesity and overweight

13. Lipids-2015 Conference
Comparision of lipids and fumonisins content in maize with diversity genetic
39 samples of maize from Morelos, State of Mexico and Hidalgo
Hybrid maize
Bt maize
Native maize

14. Chemical Analysis test
Lipids-2015 Conference
Chemical Analysis test
Total lipid content by Official method AOAC, Goldfish technique
Fatty acid methyl esters by Gaseous Cromatography
Fumonisins determination by QuickTox Fumonisins (LFD)

15. Fumonisins detection (FLD)
Lipids-2015 Conference
Fumonisins detection (FLD)

16. Fumonisins quantification
Lipids-2015 Conference
Fumonisins quantification

17. Lipids-2015 Conference
RESULTS
Fumonisin levels detected in native and improved corn hybrids are in recommendation (1.20 ppm)
Bt maize can reduce 36.66% Fumonisins contamination

18. Lipids-2015 Conference PROOF POSITIVE TO FUMONSINS RESULTS

19. MYCOTOXINS (MULTY ANALYSIS)

20. Comparison between genotypes maize
Lipids-2015 Conference
Comparison between genotypes maize

21. Lipids-2015 Conference
Profile fatty acids
Profi

22. Lipids-2015 Conference
CONCLUSIONS
There are differences in the lipid content of corn genotype
The lipid profile varies depending on the genotype and the place of origin (environmental conditions)
The improved maize increase the calories intake in Mexican diet causing overweight, diabetes and heart problems
Fumonisin was detected in all genotypes of maize evaluated
There is a risk for the consumer of corn contaminated with fumonisin metabolism of sphingolipids
It is urgent to continue clinical research on the role of fatty acids monunsaturated with increased insulin resistance

23. Thank you