LUSH PHEROMONE Manhasset Science Research Jillian Wong

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Knowledge Base Pheromones- a chemical substance secreted by many animal species that alter behavior 5R6S-6-acetoxy-5-hexadecanolide

Knowledge Base OBP-odorant binding protein University of Colorado

Literature review Rockefeller center

Literature Review Barbosa, R. MR, Souto, A., Eiras, A.E, Regis, L Laboratory and field evaluation of an oviposition trap for Culex quinquefasciatus (Diptera: Culicidae)

Literature Review.S. Ha, D.P Smith A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate-Induced Responses in Drosophila

Literature review

Literature Review

Purpose The purpose of this experiment is to measure the reaction of the Mutant lush drosophila and the regular drosophila when it comes in contact with 6-Acetoxy-5- hexadecanolide

Hypothesis Alternate Hypothesis- whenever the Mutant lush drosophila are in the presence of the pheromone they will not have a response, or they will not have a strong response to the pheromone. However, when the regular drosophila are in the presence of the pheromone, they will have a stronger reaction to it. Null Hypothesis-when the mutant Drosophila are within the presence of the drosophila, there will be no significant decrease in their reaction, and when the regular drosophila are within the presence of the pheromone there will be no significant increase in reaction.

Oviposition trap BR-OVT Protocol

The Effects of 6-acetoxy-5-hexadecanolide on drosophila melanogaster Lush mutant drosophila n=20Regular drosophila n=20 Group 2 Group 3Group 4Group 5Group 6 Group 1 Group 8Group 7 Test in oviposition trap BR-OVT with the measurements of 1, 1.5, 2, and 2.5 µl Run 80 trials, each for 10 minutes, record number of flies that are attracted to the food during each trial Compare means, then analyze using SPSS, with an ANOVA and a Scheffe-post-hoc test,

Do-ability Most of what is needed is already in the lab. The only problem is finding the pheromone.

Budget Vendor Order # quantity Description Price Total Carolina Micropipette tips (1-200 ul) yellow Wards 15 V VWR Signature variable volume Pipettors

Bibliography Department of Pharmacology and Center for Basic Neuroscience, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX , USA Ha, Tal Soo, and Dean P. Smith. "A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate Induced Responces in Drosophila." The Journal Of Neuroscience (2006): Jin, Xin, Tal Soo Ha, and Dean P. Smith. "PNAS." SNMP is a signaling component required for Pheromone Sensitivity in Drosophila 31st ser. 105 (Ausgust 5, 2008): "Pheromone." McGraw-Hill Encyclopedia of Science & Technology. 8th ed. 13 vols. Mechanicsburg: McGraw-Hill, Smith, Dean P. "Odor and Pheromone detection in Drosophila melanogaster." Pflugers Arch-Eur J Physiol (2007): University Of Texas Southwestern Medical Center At Dallas. "Researchers Find How Protein Allows Insects To Detect And Respond To Pheromones." ScienceDaily 27 January May UT Southwestern Medical Center. "Unique Pheromone Detection System Uncovered." ScienceDaily 27 June April Xu P-X, Atkinson R, Jones DNM, Smith DP (2005) Drosophila OBP LUSH is required for activity of pheromone sensitive neurons. Neuron 45: "Wake Up and Smell The Pheromones." The Rockefeller University - Laboratory of Neurogenetics and Behavior. 20 Jan