The Model Experiment

A Complex Behavior Modularize Module AModule BModule CModule D Find appropriate model system? Literature Candidate Genes Experimental Methods Lab Assays YesNo Well studied non- model example Mutants Search StudyEvaluate Analyze Generate Hypotheses and design experiments Literature Candidate Genes Experimental Methods Behavior “orthologs”

Example Insect Behavior Module AModule B Module C Module D Fly, Mouse Literature Candidate Genes Experimental Methods Lab Assays Termite Mutants Generate Hypotheses and design experiments Literature Candidate Genes Experimental Methods Fly Fly, Mouse Ortholog 1 Ortholog 2 Ortholog 3 Ortholog 4 Fly

A Complex Behavior Analyze gene expression Variant AVariant BVariant CVariant D Gene expression profiles Ortholog analysis Functional annotation Pathways Correlated expression Look for Study Meta-analyze Analyze Generate Hypotheses and design experiments Co-regulation

Example Insect Behavior Microarray analysis Population A Population B Population C Genes with different expression profiles between populations Fly and mouse orthologs Functional annotation Pathways Correlated expression Look for Study Meta-analyze Analyze Generate Hypotheses and design experiments Co-regulation

The Fantastic BeeSpace Type query Entity Identifier Module Maker Other entities Tuned by user feedback Name and number the modules, describe each module Model system Finder Behavior Ortholog Search Engine User approved User select focal model system Experiment Builder Candidate Gene Bank Mutant Base Assay Engineer Different PnPs Hypothesis Generator