The Classification Of Genus Bacillus

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Presentation transcript:

The Classification Of Genus Bacillus Liu Guohong

Content Characteristic of Bacillus Related Bacillus and Its summarization Fatty acid identification The phenotypic characteristic of ten Bacillus species

Characteristic of Bacillus Bacillus rod-shaped, Gram-positive or variable, spore-forming, aerobic or facultatively anaerobic. The genus includes thermophilic and psychrophilic, acidophilic and alkalophilic, freshwater and halophilic bacteria that utilize a wide range of carbon sources for heterotrophic growth or grow autotrophically.

Colony roough, untransparent, cream-white or brown, drape, producing pigment. pH5.5-8.5. G+C 41.5-47.5 mol%. Type species is Bacillus.subtilis.

Related Bacillus and Its summarization

Thermobacillus Gram-negative, rod-shaped, aerobic,spore-forming, non-motile, Main fatty acid is iso-C16:0. The G+C is 57.5 mol%. Type species is Thermobacillus xylanilytus. Geobacillus Vegetative cell rod-shaped, single or in short chain, motile by means of peritrichous flagella or non-motile aerobic or facultatively anaerobic. 37-75 ℃, pH6.0-8.5. Main fatty acid is iso-C15:0, iso-C16:0 and iso-C17:0. The G+C is 48.2-58 mol%. Type species is Geobacillus stearothermophilus.

Ureibacillus Colony cirluar,flat, transparent,motile,Gram-negative, rod-shaped or in chain, aerobic, thermopfilic. Main fatty acid is iso-C16:0. The G+C is 35.7-41.5 mol%. Type species is Ureibacillus thermosphaericus.

Thiobacillus Growth condition range is large, pH0-8, 20-50℃. Colony is small, Gram-negative, motile. G+C 50-68%.

The second edition of《Bergey's Manual of Systematic Bacteriology》, eight species of Thiobacillus were reassigned to three new genera，namely Acidithiobacillus (including Thiobacillus thiooxidans, Thiobacillus ferrooxidans, Thiobacillus caldus and Thiobacillus albertensis), Halothiobacillus (including Thiobacillus neapolitanus, Thiobacillus halophilus and Thiobacillus hydrothermalis) and Thermithiobacillus (including Thiobacillus tepidarius).

Halothiobacillus Gram-negative,rod-shaped, Obligately acidophilic, using reduced sulfur compounds to support autotrophic growth. optimum tempaerate is 30-40 ℃, pH6.5-8.0 . The G+C is 56-68%. Type species is Halothiobacillus neapolitanus (formerly Thiobacillus neapolitanus) Thermithiobacillus Obligately aerobic, moderately thermophilic and chemolithoautotrophic, Gram-negative, non-sporeforming Grow on reduced inorganic sulfur compounds;, unable to oxidize ferrous iron. G+C 66-67%. The type species is Thermithiobacillus tepidarius (formerly Thiobacillus tepidarius). Acidithiobacillus Obligately chemolithoautotrophic Gram-negative, rods, obtaining energy from reduced inorganic sulfur compounds. G+C 52-64 mol%， The type species is Acidithiobacillus thiooxidans (formerly Thiobacillus thiooxidans).

Gracilibacillus Obligately aerobic. Main fatty acid is anteiso15:0, The G+C is 38 -39 mol%. The type species is Gracilibacillus halotolerans. Salibacillus The main fatty acid is iso15:0 and anteiso 15:0. The type species is Salibacillus salexigens. Brevibacillus Rod-shaped,Gram-positive or variable，obligately aerobic. Main fatty acid is iso-C15:0 and anteiso-C15:0, The G+C is 42.8-57.4 mol%. The type species is Brevibacillus brevis.

Aneurinibacillus Main fatty acid is iso-C15:0, iso-C16:0and C16:0 , The G+C is 41.1-43.4 mol%. The type species is Aneurinibacillus aneurinolyticus. Lentibacillus Main fatty acid is anteiso-C15:0 and iso-C16:0. The G+C is 44 mol%. The type species is Lentibacillus salicampi. Jeotgalibacillus Main fatty acid is iso-C15:0. G+C 44 mol%. The type species is Jeotgalibacillus alimentarius. Marinibacillus Main fatty acid is anteiso-C15:0. The type species is Marinibacillus marinus.

Methylobacillus Main fatty acid is C16:0 and C16:1. Paralactobacillus G+C 46.1-46.2 mol% The type species is Paralactobacillus selangorensis. Panenibacillus Main fatty acid is anteiso-C15:0. Anoxybacillus G+C 42 mol%. The type species is Anoxybacillus pushchinensis. Virgibacillus Main fatty acid is iso-C15:0 and anteiso-C15:0.

Fatty acid identification

The phenotypic characteristic of ten Bacillus species

菌名 megaterium 4617 Bacillus Bacillus megaterium 4632 subtilis 4620 licheniformis 4648 licheniformis 4646 pumilus4657 cereus4645 Bacillus cereus 4634 atrophaeus 4643 sphaericus 4639 耐盐2% + 5% 7% - 10% 温度5 +- 10 50 55 pH5.7 V-P 接触酶淀粉水解

Thanking for your attention