Cultured in vitro human autologous epidermis for clinical applications Justyna Drukała Laboratory of Cell and Tissue Engineering Department of Cell Biology Faculty of Biochemistry, Biophysics & Biotechnology Jagiellonian University

Skin-replecement product technology 0.5 cm2 patients own skin biopsy epidermal cells culturing in vitro prepared cells transplanted back on to the same patient in an injectable fibrin matrix after 2 weeks of in vitro cell culture applicated cells are capable of dividing with high growth potential, form growing epidermal islands in wound bed

Primary culture of epidermal cells after enzymatic dissociation

Posibilities of our method utilization This skin-replacement product is designed to meet the needs mainly of the chronic ulcers and burns care

Keratinocytes cell suspension 3 weeks after Keratinocytes cell suspension in fibrin-glue cells transplantation 3 months after

Technology advantages no rejection by patient – permanent wound covering no adverse reactions in the clinic no associated infections no risk of disease transmission significantly better wound closure efficancy in non-healing wounds than by using conventional noninvasive methods scarless wound healing

Other skin-repacement matherials Split-skin autotransplantation (require substantially large skin flaps – under general anesthesis) Allografts or other biological dressing - temporal wound covering

Current status development of strategic alliances for the promotion of the product searching for substantial funding for the venture