Supplemental Figures, for reviews only. MNV RdRp RNA Formadehyde MNV RdRp+ PS, X-link MNV RdRp+ Hp, X-link MNV RdRp+ PS, no X-link MNV RdRp+ Hp, no X-link.

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Supplemental Figures, for reviews only. MNV RdRp RNA Formadehyde MNV RdRp+ PS, X-link MNV RdRp+ Hp, X-link MNV RdRp+ PS, no X-link MNV RdRp+ Hp, no X-link m/z Intensity (A.U. x 10 3 ) ✓ ✓ ✓ ✓ ✓ ✓ Mps ✓ Mph Mps Mph ✓ sFig. 1. MALDI-ToF spectra use for analysis of the RCAP data. The conditions used are listed to the right of the spectra. Mps is the MNV proscript, Mph is the hairpin portion of the Mps. Formaldehyde is used to crosslink protein to RNA.

Mhp 2-4SU 15-4SU 24-4SU Ratio, WT Mph: competitor Relative RNA synthesis sFig. 2. RNA hairpins from the MNV proscripts that contain 4-thio- uridine modifications are recognized by the MNV RdRp in an identical manner as the unmodified Mph. The results are the summary of the amount of RNA products made from Mps in the presence of no competitor or the four competitors, Mph, 2-4SU, 15-4SU, and 24-4SU.

KR(4SU)LLWGSDLGTMIR WDSTQQR(4SU)AILK(4SU) Intensity sFig. 3. Mass spectra identifying the residues that contacted the 4-SU in the RNA 2-4SU and 24-4SU. The crosslinked peptides were identified using mass spectrometry on an LTQ-Orbitrap instrument. A) High-resolution mass spectrum (MS) of the peptide ion [KR(4SU)LLWGSDLGTMIR – S] +4 (RdRp residues ) from the reaction with 24-2SU RNA. The measured monoisotopic mass (5 ppm mass error) is at m/z (+4). (Middle) CID spectrum of this peptide ion at m/z The CID fragment ion assignments are illustrated with the dashed lines in the below structure. (Bottom) Proposed structure and CID fragments (dashed lines) of the singly- substituted 4SU peptide. The most common loss upon fragmentation is that of the ribose sugar (m/z 133) from either the parent ion or its “b ion” peptide fragments. B) (Top) High- resolution mass spectrum (MS) of the peptide ion [WDSTQQR(4SU)AILK(4SU) – O 2 ] +2 (RdRp residues ) from the reaction with 2-2SU substituted RNA. The measured monoisotopic mass (5 ppm mass error) is at m/z (+2). (Middle) CID spectrum of this peptide ion at m/z D) The CID fragment ion assignments are illustrated with the dashed lines in the below structure. (Bottom) Proposed structure and CID fragments (dashed lines) of the doubly- substituted 4SU peptide. Unlike the other two peptides, this peptide has lost two oxygen atoms from both of its ribose moieties and its most common loss upon fragmentation is that of the deoxygenated ribose sugar (m/z 213) from its “b and y ion” peptide fragments. AB

M R185A R255A K259A MNV RdRps Fold, DN product Fold, PE product MNVps conc. (nM) A B sFig. 4. RNA synthesis by mutant MNV RdRps R185A, R255A, and K259A. A) Gel image of the recombinant proteins used in this analysis. B) Summary of the amount of de novo-initiated products (upper panel) and the primer-extension (PE) products (lower panel) made by the WT and mutant MNV RdRps. Mutant R259A was unaffected for both modes of RNA synthesis. Mutants R185A and R255A were both defective for primer extension and de novo initiated RNA synthesis.