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Beam-type (Q-TOF) CID data of m/z (3+).

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Presentation on theme: "Beam-type (Q-TOF) CID data of m/z (3+)."— Presentation transcript:

1 Beam-type (Q-TOF) CID data of m/z 815.73(3+).
Beam-type (Q-TOF) CID data of m/z (3+). From these data one could identify the modified peptide as AVGAQVLESTPPPHVMR ([682–698] of bovine ITIH2 protein (Uniprot ID: F1MNW4)). The glycan composition can be “guessed” from the oxonium ions of HexNAc and Neu5Ac, and from the presence of m/z (2+) as Neu5AcHexHexNAc. Intensity pattern of the fragments of the HexNAc oxonium ion (m/z 204) ascertain its identity as GalNAc (89). Thus, the sugar structure is most likely Neu5AcGalGalNAc. Its linearity or branching cannot be determined, because larger Y fragments were not detected, nor were m/z or observed, indicating sialylation of Gal or GalNAc, respectively. The site of modification cannot be assigned. All ions that were gas-phase deglycosylated, and thus, were detected unmodified are printed in blue. Fragment y8 belongs to this series only if Thr-10 is the modification site. Zsuzsanna Darula, and Katalin F. Medzihradszky Mol Cell Proteomics 2018;17:2-17 © 2018 by The American Society for Biochemistry and Molecular Biology, Inc.

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