EXPERIMENTAL PATHWAYS 1.What ’ s the Cyclins? 2.What ’ the relationship between cyclins and MPF?

What’s the cyclins? 一、 MPF 一、 MPF 卵细胞促成熟因子， matuation- promoting factor ； 卵细胞促成熟因子， matuation- promoting factor ； 细胞促分裂因子， mitosis-promoting factor ； M 期促进因子， M-phase-promoting factor ）。

二、 Cyclins 细胞周期素 二、 Cyclins 细胞周期素 yeast: Cln1,Cln2,Clin3,Clb1-Clb6 Vertabrate: A1-2 、 B1-3 、 C 、 D1- 3 、 E1-2 、 E 、 F 、 G 、 H 等

Every cyclins has a 100 amides keep sequence, which called cyclin-frame.It can interpose cyclin combine with CDK(cycling dependent kinase), different cyclins will recognize different CDK to make a cycling- complex. M-

周期蛋白激酶 CDK ( cyclin dependent kinase),

Different cycling expression in different time and combine with different CDK to accommodate different CDKase activition.

注射实验表明： 1. 孕酮诱导卵母细胞成熟； 1. 孕酮诱导卵母细胞成熟； 2. 成熟卵细胞质中，含有卵母细胞成熟 的因子，称做 MPF 2. 成熟卵细胞质中，含有卵母细胞成熟 的因子，称做 MPF

P619 Kinetics of Xenopus oocyte activation by progesterone and cyclinA mRNA Kinetics of Xenopus oocyte activation by progesterone and cyclinA mRNA GVBD%GVBD%

What’s the relationship between cycling and MPF? cyclinA is an activator of MPF cyclinA is an activator of MPF MPF=CDC2+Cyclin B =a complex MPF=CDC2+Cyclin B =a complex MPF

Cyclin B 的降解途径

P34cdc2 The yeast protein was called p34cdc2 or simply cdc2. The yeast protein was called p34cdc2 or simply cdc2. The first evidence of a link between cdc2 and MPF came as the result of a collaboration between yeast and amphibian research group The first evidence of a link between cdc2 and MPF came as the result of a collaboration between yeast and amphibian research group

Recall from fission yeast were shown to react specifically with the 32- and 45-kDa protein Antibodies formed against cdc2 from fission yeast were shown to react specifically with the 32-kDa component of MPF isolated from Xenpus eggs. This component of MPF is a homologue of the 34-kDa yeast kinase and, the machinery controlling the cell cycle in yeast and vertebrates contains evolutionarily conserved components.

2001 年 10 月 8 日, L. Hartwell 、 P. Nurse 、 T. Hunt 因对细胞周期调控机理的研究而荣获诺贝 尔生理医学奖。