TEIN 4: RNA-seq analysis Akzam Saidin Sr. Bioinformatics Scientist.

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TEIN 4: RNA-seq analysis Akzam Saidin Sr. Bioinformatics Scientist

RNA Transcription and Processing

Gene Structure and Alternative Splicing

2015 NOVOCRAFT TECHNOLOGIES. PROPRIETARY AND CONFIDENTIAL.

Alignment Accuracy Trial 1Trial 2

Figure from Wang et. al, RNA-Seq: a revolutionary tool for transcriptomics, Nat. Rev. Genetics 10, 57-63, 2009.

2015 NOVOCRAFT TECHNOLOGIES. PROPRIETARY AND CONFIDENTIAL.

References 1. KR Kukurba and SB Montgomery. RNA Sequencing and Analysis Cold Spring Harb Protoc; doi: /pdb.top M Griffith, JR Walker, NC Spies, BJ Ainscough, OL Griffith. Informatics for RNA Sequencing: A Web Resource for Analysis on the Cloud PLOS Computational Biology. DOI: /journal.pcbi Z Wang, M Gerstein and M Snyder. RNA-Seq: a revolutionary tool for transcriptomics Nat Rev Genet. 10(1): doi: /nrg NOVOCRAFT TECHNOLOGIES. PROPRIETARY AND CONFIDENTIAL.