1. Testis Restricted Expression and Alternative Splicing of SVA-derived Transcripts of MRGPRX3 Gene
Yu-Na Noh1, Jae-Won Huh2, Dae-Soo Kim3, Hong-Seok Ha1, Kung Ahn1 and Heui-Soo Kim1
1. Division of Biological Science, College of Natural Sciences, Pusan National University, Busan , Republic of Korea
2. National Primate Research Center (NPRC), KRIBB, Cheongwon , Republic of Korea
3. Korea Bioinformation Center, KRIBB, Daejeon  , Republic of Korea
Abstract
MRGPRX3 [MAS-related GPR (G protein-coupled receptor) member X3] gene located on chromosome 11p15.1 region, and MRGPRX3 gene were as known as sensory neuron specific receptors that detect painful stimuli and associated with modulation of nociception. MRGPRX3 gene has alternative transcripts which are SVA-derived transcripts and original transcripts. Using experimental approaches and computational screening, we identified three SVA-derived and two original transcripts. Intriguingly, three SVA-derived transcripts are expressed specifically in testis tissues. However, original transcript is corresponding to transcribe ubiquitously in several tissues. Integration lineage of SVA element was analyzed by PCR with various primate DNA samples. The results indicated that SVA element in MRGPPRX3 gene has been integrated into our common ancestor genome after the divergence of human and chimpanzee lineage. Taken together, integration event of SVA element in MRGPRX3 gene during the primate evolution seems to contribute the diversity of human transcriptome by providing testis specific
promoter and following alternative transcripts.
Introduction
Results and Discussion
[ Figure 1. Alternative transcripts of MRGPRX3 gene ]
[Structure of Retrovirus]
[Cycle of Retrovirus]
[Figure 2. Integration lineage of SVA element during the Primate evolution.]
[Structure of SVA ( SINE (short interspersed element), VNTR(variable number of tandem repeat) and Alu)]
[Figure 3. RT-PCR analysis of MRGPRX3 gene transcript]
[Amplification dynamics of the SVA family of retroposons in primates. Putative evolutionary history of the SVA lineage in primate genomes.]
[Figure 4. Sequence alignment of MRGPRX3 gene transcript]
Materials and Methods
[Figure 5. Alternative transcripts of MRGPRX3 gene by SVA]
PCR
Primate DNA
MRGPRX3 Gene
References
Hui Wang et al., 2005, SVA Elements: A Hominid-specific Retroposon Family, Journal of Molecular Biology,Vol.354:994–1007
Satoshi Makino et al., 2007, Reduced Neuron-Specific Expression of the TAF1 Gene Is Associated with X-Linked Dystonia-Parkinsonism, The American Journal of Human Genetics, Vol.80,
Ryan E. Mills et al., 2007, Which transposable elements are active in the human genome?, TRENDS in Genetics, Vol.23,
Gene
Cloning
Bio
Informatics
Amplified
Plasmid DNA