Isolation and Enrichment of soil bacteria Regina Gohl, K. Noreen Boyle 02/11/2011 University of Oldenburg - ICBM - Practical course Physiology of bacteria (Group B1) – final presentation

Introduction  Aims & Expectations  Course results  Summary Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 2 Introduction 1 soil probe  2 experiments Isolation Enrichment ? 37 °C Pasteurisation Nitrogen Nitrogen + yeast Salt Salt+yeast Acidic Alkaline Dilution series Medium variation Introduction  Aims & Expectations  Course results  Summary

Introduction  Aims & Expectations  Course results  Summary Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 3 Aims and expectations Cultivation of soil bacteria Determination of abundance Comparison: fast vs abundant Influence of different media Expectations Direct isolation Enrichment Types of bacteria several one - few Colony size small large Growth slow fast Conclusion abundant fast growing Introduction  Aims & Expectations  Course results  Summary

Introduction  Aims & Expectations  Course results  Summary Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 4 Direct Isolation Cells/g soil 3.1 x 10^6 5.4 x 10^6 3.3 x 10^6 1.9 x 10^6 4.7 x 10^6 1.0 x 10^6 0.8 x 10^6 ~ 10^9 bacteria/g soil 2.8 x 10^6 cells/g soil ~1 % of bacteria can be cultured in the lab Less bacteria than expected Introduction  Aims & Expectations  Course results  Summary

Introduction  Aims & Expectations  Course results  Summary Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 5 Direct Isolation Introduction  Aims & Expectations  Course results  Summary

Introduction  Aims & Expectations  Course results  Summary Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 6 Enrichment Treatment Oxidase test Gram test Catalase test Increased temperature + - Pasteurisation - nitrogen source -nitrogen source + yeast extract + salt + salt + yeast extract acidic alkaline http://www.laborwissen.de/wiki/ tiki-index.php?page=Katalase-Test Introduction  Aims & Expectations  Course results  Summary

Introduction  Aims & Expectations  Course results  Summary Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 7 Enrichment Treatment Motility Growth Size Shape Increased temperature mobile fast large round, beige Pasteurisation immobile slow small - nitrogen source immobile, rods white, smooth + yeast extract yellow, smooth + salt - small, round, white + salt + yeast extract large, round, white acidic cocci, immobile beige, bright alkaline Introduction  Aims & Expectations  Course results  Summary

Introduction  Aims & Expectations  Course results  Summary Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 8 Summary Results as expected Variety of bacteria in soil Different treatment  growth / no growth - nitrogen source + yeast extract - nitrogen source Introduction  Aims & Expectations  Course results  Summary

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