1. Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial Cultures Culture Media Measurement of Growth FINAL ROUND

2. Topic 1: Physical Requirements $100 Question The optimum pH for most bacteria is a. 1 b. 5 c. 7 d. 9 BACK TO GAME ANSWER

3. Topic 1: Physical Requirements $100 Answer The optimum pH for most bacteria is a. 1 b. 5 c. 7 (correct answer) d. 9 BACK TO GAME

4. Topic 1: Physical Requirements $200 Question A psychrophile grows best at a. 37°C b. 0°C c. 25°C d. 15°C BACK TO GAME ANSWER

5. Topic 1: Physical Requirements $200 Answer A psychrophile grows best at a. 37°C b. 0°C (correct answer) c. 25°C d. 15°C BACK TO GAME

6. Topic 1: Physical Requirements $300 Question Organisms that require high salt concentrations for growth are called a. Halophiles b. Extreme halophiles c. Facultative halophiles d. Microhalophiles BACK TO GAME ANSWER

7. Topic 1: Physical Requirements $300 Answer Organisms that require high salt concentrations for growth are called a. Halophiles b. Extreme halophiles (correct answer) c. Facultative halophiles d. Microhalophiles BACK TO GAME

8. Topic 1: Physical Requirements $400 Question The energy source utilized by producers in the hydrothermal vents on the ocean floor is a. Light b. Sulfur c. Organic molecules d. Carbon dioxide BACK TO GAME ANSWER

9. Topic 1: Physical Requirements $400 Answer The energy source utilized by producers in the hydrothermal vents on the ocean floor is a. Light b. Sulfur (correct answer) c. Organic molecules d. Carbon dioxide BACK TO GAME

10. Topic 1: Physical Requirements $500 Question In food preservation, salts and sugars are used because they create a a. Lower pH b. Hypertonic environment c. Hypotonic environment d. Lower osmotic pressure BACK TO GAME ANSWER

11. Topic 1: Physical Requirements $500 Answer In food preservation, salts and sugars are used because they create a a. Lower pH b. Hypertonic environment (correct answer) c. Hypotonic environment d. Lower osmotic pressure BACK TO GAME

12. Topic 2: Chemical Requirements $100 Question Chemoautotrophs and photoautotrophs derive their carbon from a. Carbon dioxide b. Proteins c. Lipids d. Carbohydrates BACK TO GAME ANSWER

13. Topic 2: Chemical Requirements $100 Answer Chemoautotrophs and photoautotrophs derive their carbon from a. Carbon dioxide (correct answer) b. Proteins c. Lipids d. Carbohydrates BACK TO GAME

14. Topic 2: Chemical Requirements $200 Question Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called a. Chemosynthesis b. Photosynthesis c. Nitrogen fixation d. Nitrogen synthesis BACK TO GAME ANSWER

15. Topic 2: Chemical Requirements $200 Answer Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called a. Chemosynthesis b. Photosynthesis c. Nitrogen fixation (correct answer) d. Nitrogen synthesis BACK TO GAME

16. Topic 2: Chemical Requirements $300 Question An organism that uses oxygen but can grow without oxygen is called a(n) a. Obligate aerobe b. Facultative aerobe c. Aerobe d. Aerotolerant anaerobe BACK TO GAME ANSWER

17. Topic 2: Chemical Requirements $300 Answer An organism that uses oxygen but can grow without oxygen is called a(n) a. Obligate aerobe b. Facultative aerobe (correct answer) c. Aerobe d. Aerotolerant anaerobe BACK TO GAME

18. Topic 2: Chemical Requirements $400 Question This organism is killed by atmospheric oxygen: a. Aerobe b. Obligate aerobe c. Obligate anaerobe d. Aerotolerant anaerobe BACK TO GAME ANSWER

19. Topic 2: Chemical Requirements $400 Answer This organism is killed by atmospheric oxygen: a. Aerobe b. Obligate aerobe c. Obligate anaerobe (correct answer) d. Aerotolerant anaerobe BACK TO GAME

20. Topic 2: Chemical Requirements $500 Question Biofilms reside in a matrix made up primarily of a. Lipids b. Hydrogel c. Proteins d. Polysaccharides BACK TO GAME ANSWER

21. Topic 2: Chemical Requirements $500 Answer Biofilms reside in a matrix made up primarily of a. Lipids b. Hydrogel c. Proteins d. Polysaccharides (correct answer) BACK TO GAME

22. Topic 3: Growth of Bacterial Cultures $100 Question Generation time can best be defined as a. The length of time it takes for lag phase b. The duration of log phase c. The length of time it takes for a cell to divide d. The minimum rate of doubling BACK TO GAME ANSWER

23. Topic 3: Growth of Bacterial Cultures $100 Answer Generation time can best be defined as a. The length of time it takes for lag phase b. The duration of log phase c. The length of time it takes for a cell to divide (correct answer) d. The minimum rate of doubling BACK TO GAME

24. Topic 3: Growth of Bacterial Cultures $200 Question To cultivate anaerobic bacteria, one must use special media called a. Reducing media b. Oxidizing media c. Differential media d. Selective media BACK TO GAME ANSWER

25. Topic 3: Growth of Bacterial Cultures $200 Answer To cultivate anaerobic bacteria, one must use special media called a. Reducing media (correct answer) b. Oxidizing media c. Differential media d. Selective media BACK TO GAME

26. Topic 3: Growth of Bacterial Cultures $300 Question Capnophiles must be grown in high concentrations of a. Oxygen b. Salt c. Glucose d. Carbon dioxide BACK TO GAME ANSWER

27. Topic 3: Growth of Bacterial Cultures $300 Answer Capnophiles must be grown in high concentrations of a. Oxygen b. Salt c. Glucose d. Carbon dioxide (correct answer) BACK TO GAME

28. Topic 3: Growth of Bacterial Cultures $400 Question The culture medium that is designed to increase numbers of desired microbes to detectable levels is called a. Complex b. Selective c. Enrichment d. Differential BACK TO GAME ANSWER

29. Topic 3: Growth of Bacterial Cultures $400 Answer The culture medium that is designed to increase numbers of desired microbes to detectable levels is called a. Complex b. Selective c. Enrichment (correct answer) d. Differential BACK TO GAME

30. Topic 3: Growth of Bacterial Cultures $500 Question The culture medium that is designed to suppress unwanted microbes while encouraging desired microbes is called a. Complex b. Selective c. Reducing d. Differential BACK TO GAME ANSWER

31. Topic 3: Growth of Bacterial Cultures $500 Answer The culture medium that is designed to suppress unwanted microbes while encouraging desired microbes is called a. Complex b. Selective (correct answer) c. Reducing d. Differential BACK TO GAME

32. Topic 4: Culture Media $100 Question To neutralize acids as they are produced in a culture, what should be added to the culture medium? a. Buffers b. Salts c. Sugars d. PABA BACK TO GAME ANSWER

33. Topic 4: Culture Media $100 Answer To neutralize acids as they are produced in a culture, what should be added to the culture medium? a. Buffers (correct answer) b. Salts c. Sugars d. PABA BACK TO GAME

34. Topic 4: Culture Media $200 Question The microbes that grow and multiply in or on a culture medium are referred to as a(n) a. Culture b. Inoculum c. Specimen d. Medium BACK TO GAME ANSWER

35. Topic 4: Culture Media $200 Answer The microbes that grow and multiply in or on a culture medium are referred to as a(n) a. Culture (correct answer) b. Inoculum c. Specimen d. Medium BACK TO GAME

36. Topic 4: Culture Media $300 Question What can be added to a medium when it is desirable to grow bacteria on a solid medium? a. Glucose b. Magnesium sulfate c. Potassium phosphate d. Agar BACK TO GAME ANSWER

37. Topic 4: Culture Media $300 Answer What can be added to a medium when it is desirable to grow bacteria on a solid medium? a. Glucose b. Magnesium sulfate c. Potassium phosphate d. Agar (correct answer) BACK TO GAME

38. Topic 4: Culture Media $400 Question A culture medium consisting of agar, human blood, and beef heart is a a. Complex medium b. Selective medium c. Differential medium d. Chemically defined medium BACK TO GAME ANSWER

39. Topic 4: Culture Media $400 Answer A culture medium consisting of agar, human blood, and beef heart is a a. Complex medium (correct answer) b. Selective medium c. Differential medium d. Chemically defined medium BACK TO GAME

40. Topic 4: Culture Media $500 Question The source of nutrients in nutrient agar is a. Agar b. Peptone and sodium chloride c. Peptone and beef extract d. Nutrient BACK TO GAME ANSWER

41. Topic 4: Culture Media $500 Answer The source of nutrients in nutrient agar is a. Agar b. Peptone and sodium chloride c. Peptone and beef extract (correct answer) d. Nutrient BACK TO GAME

42. Topic 5: Measurement of Growth $100 Question A spectrophotometer can be used to measure a. Metabolic activity b. Turbidity c. Dry weight d. Pathogenicity BACK TO GAME ANSWER

43. Topic 5: Measurement of Growth $100 Answer A spectrophotometer can be used to measure a. Metabolic activity b. Turbidity (correct answer) c. Dry weight d. Pathogenicity BACK TO GAME

44. Topic 5: Measurement of Growth $200 Question Because of time considerations, which method is usually used to count the number of bacteria in milk? a. The direct microscope count method b. The pour plate method c. The spread plate method d. The most probable number method BACK TO GAME ANSWER

45. Topic 5: Measurement of Growth $200 Answer Because of time considerations, which method is usually used to count the number of bacteria in milk? a. The direct microscope count method (correct answer) b. The pour plate method c. The spread plate method d. The most probable number method BACK TO GAME

46. Topic 5: Measurement of Growth $300 Question Which method is utilized to ensure that plates containing 25 to 250 colonies are counted? a. The most probable number method b. Serial dilution c. Filtration d. Spread plate method BACK TO GAME ANSWER

47. Topic 5: Measurement of Growth $300 Answer Which method is utilized to ensure that plates containing 25 to 250 colonies are counted? a. The most probable number method b. Serial dilution (correct answer) c. Filtration d. Spread plate method BACK TO GAME

48. Topic 5: Measurement of Growth $400 Question Microorganisms are particularly sensitive to adverse conditions during the a. Lag phase b. Death phase c. Stationary phase d. Log phase BACK TO GAME ANSWER

49. Topic 5: Measurement of Growth $400 Answer Microorganisms are particularly sensitive to adverse conditions during the a. Lag phase b. Death phase c. Stationary phase d. Log phase (correct answer) BACK TO GAME

50. Topic 5: Measurement of Growth $500 Question Which of the following metabolic activities can be used to measure microbial growth? a. Carbon dioxide consumption b. Oxygen consumption c. Glucose consumption d. Salt consumption BACK TO GAME ANSWER

51. Topic 5: Measurement of Growth $500 Answer Which of the following metabolic activities can be used to measure microbial growth? a. Carbon dioxide consumption b. Oxygen consumption c. Glucose consumption (correct answer) d. Salt consumption BACK TO GAME

52. FINAL ROUND Question An experiment began with 5 cells and ended with 160 cells. How many generations did the cells go through? a. 20 b. 6 c. 4 d. 5 BACK TO GAME ANSWER

53. FINAL ROUND Answer An experiment began with 5 cells and ended with 160 cells. How many generations did the cells go through? a. 20 b. 6 c. 4 d. 5 (correct answer) BACK TO GAME