Introduction to training Agenda Product overview What’s new from ProStar 325 Hardware overview Initialization routine Software CD overview Detector installation & PolyVerfiy PolyDiagnostics

Product Overview Based on the ProStar 325 with many shared parts Two models available: Single 1024 array Dual 1024 array Control by both Star with PolyView 2000 and Galaxie (PolyView is now included with Star driver)

Product Overview - workstation control Galaxie Four 335 DADs per acquisition server Driver included in Varian LC control drivers Star two 335 DADs per Star Workstation Driver included in LC control option Installation of driver automatically installs PolyView 2000 Ethernet communications best with a switch (not a hub)

ProStar 335 and 325 similarities Product Overview ProStar 335 and 325 similarities Same cabinet Same UV & visible lamps Same flow cells Same rear panel, connectors, interface boards & cables Same communications protocol Same power supply Same Main pwb (difference in firmware)

What’s New Same look as ProStar 325 except no PPC capabilities

Different optics module What’s New Different optics module Dual Array 1024 photodiode sample and reference array Extended range spectra Used for Analytical to Prep Applications Single Array 1024 photodiode sample array 2 photodiode references (250 and 500 nm) No extended range Uses 9x0 and 4x0 flow cells only

Hardware Overview – optics module

Product Overview – ray trace diagram

Hardware Overview - Polychromator Available slit widths 1nm 2nm 4nm 8nm 16nm

Hardware Overview – dual path flowcell Illustration is of a 9x1 flowcell reference sample

Hardware Overview – optics module Wavelength calibration data must be entered into the EEPROM using Diagnostics software.

Electronics – wavelength calibration factors Use the floppy disk to update the EEPROM automatically This label will be attached to the top of each optics module

Hardware Overview – Electronics Only difference between 335 & 325 is firmware

Hardware Overview - Electronics Add new MAC address label when swapping pwb’s New MAC address label comes with each new PWB

Hardware Overview – initialization routine Peak search for 656.1nm deuterium emission line Determines dark current table (0%T) Gain (Lamp) calibration is now user driven. Stored in non-volatile memory.

Initialization Hardware fault initialized Status LED’s not initialized

Software CD overview Software CD ships with each ProStar 335 detector. Part number: 85 102114 00 Auto-loading CD which contains: Galaxie drivers and manuals Star driver and manuals PolyVerfiy software PolyDiagnostics

Customer software CD 85-1xxxxx-00 will contain Software CD overview Customer software CD 85-1xxxxx-00 will contain

Software CD overview – installation PolyVerify After installing, a desktop icon will be created called ProStar Use PolyVerify to check the performance of the ProStar 335

Software CD overview - installation PolyVerify Only two test are required at installation; Noise/Drift Wavelength accuracy IQ/OQ is also available part number: 03-914756-00;4

Software CD overview – installation PolyVerify Print customer certificate

The ProStar 335 must be initialized and have both lamps on for at least one hour prior to running any tests. Noise test : pump water for at least 10 minutes at a pump head pressure of at least 100 atm using a back pressure restrictor of 40psi. Wavelength accuracy: same conditions as the Noise test.

Software CD overview - PolyDiagnostics Installing PolyDiagnostics Browse the CD using Windows Explorer. Diagnostics is not self loading and not intended for customer use.

Software CD overview - PolyDiagnostics To run PolyDiagnostics you will need to copy these three files to the ProStar directory where PolyVerify is installed. From there double click the “PolyDiagnostics.exe” file using Windows Explorer.

Software CD overview - PolyDiagnostics Similar appearance to 325 Diagnostics

Software CD - PolyDiagnostics Use the Diagnostics program to update the EEPROM and perform various calibration routines i.e. wavelength & lamp calibrations Service folder also contains the firmware updater program

Software CD - PolyDiagnostics The EEPROM contents will need updating whenever; the Main PWB is replaced the optics module is replaced to update installation & maintenance dates

Software CD - PolyDiagnostics EEPROM contents; Detector serial number MAC address Wavelength calibration factors, slope and offset IP address Gateway address Subnet mask Lamp timer (on/off date and times) Detector installation date Last maintenance date Error log Hg wavelength calibration date

Software CD - PolyDiagnostics When to calibrate Performing a Lamp Calibration will help reduce noise and improve linearity. Deuteriunm lamp is changed Visible lamp is changed Every 1000 lamp source hours Increasing signal noise When changing flowcell to one of a different pathlength