Observing and Measuring Cells Lab
1. Measure Using Reticule Reticule- ruler within the ocular lens Use to measure specimen observing under the microscope Length Reticule Measurement (400x):_________ Width Reticule Measurement (400x):________
2. Use the conversion table provided to determine µm Reticule Measurement:_________ How to use the table! Get the # of A, B, & C’s to add up to your total reticule measurement Example: Reticule Meas. = 1.6 1 C + 1 B + 1 A C 1 A B Objective Lens Power Microscope Magnification A B C Scanning 40x 25um 125 um 250 um Low 100x 10um 50 um 100 um High 400x 2.5um 12.5 um 25 um Oil 1000x 1um 5 um 10 um
3. Convert to mm (life size!) Bigger- move decimal point to the left Unit Deci 0.1 (d) Centi 0.01 (c) Milli 0.001 (m) Decimilli 0.0001 (dm) Centimilli 0.00001 (cm) Micro 0.0000001 (µ) Smaller- move decimal point to the right Multiply um by _________________ to get mm, or move the decimal place over ____ spaces. Table Converted Reticule Length:___________ This gives you the Life Size of the object
Calculating Drawing Magnification (Length Only!) 100 ÷largest life size Example: 100 ÷ __________ = Round to workable # __________________ Written as _____________ x L.S.
Calculate Drawing Dimensions Life size x drawing magnification ____________ x ____________ These are the dimensions that you will draw Include labels (follow the rubric)
Width Calculations Only determine life size Do NOT calculate magnification Use the magnification from length
Cell Drawing
Cell Drawing Within 100mm x 100 mm box, measure and draw the length & width of the object (mm) Include organelles you could see under the microscope Follow rubric provided