1. Microscope
Basics

2. Some Types of Microscopes
1. Dissecting or Stereomicroscope
used to observe specimens like insects & flowers
Object is opaque
two oculars
3d image not reversed
2. Compound light microscope
allows light to pass through
uses two lenses form image
1 ocular and light source
We will use a compound light microscope

3. Microscope Care Always carry with 2 hands
Only use lens paper for cleaning
Do not force knobs
Always store covered
Keep objects clear of desk and cords
Teacher demonstrates how to hold the microscope, where the lens paper is located and how to use it. Students will be invited to turn the knobs and observe the stage as it moves up and down. Teacher will demonstrate how to store the microscope.

4. Ocular lens (Eyepiece) – 10X magnification 1. Label the microscope
Body Tube
Nosepiece
Arm
Objectives
Stage
Stage Clips
Coarse Adjustment
Diaphragm
Fine Adjustment
Light
Base
Always carry a microscope with one hand holding the arm & one hand under the base.

5. Fill in the table on your worksheet.
2-3: What’s my power?
To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective.
What are the powers of magnification for each of the objectives we have on our microscopes?
Fill in the table on your worksheet.

6. Power of Magnification
Band Color
Objective Power
Eyepiece Lens Power
Power of Magnification
Red
Yellow
Blue 4x
10x
40x
10x
10x
100x
10x
400x
40x

7. 4. Comparing Powers of Magnification
We can see better details with higher the powers of magnification, but we cannot see as much of the image.
Which of these images would be viewed at a higher power of magnification?
Is it best to always use high power? NO!!!!!!!
5. Why can’t you use the largest objective (highest magnification) for some slides?
Sometimes there is not enough room and you will not be able to see it!

8. 6. How to Measure length with a Scope
1. Determine field of view width
2. Estimate how much of the field the object takes up
3. Divide to get length of object
4. Conversion Hint: 1 mm = 1000 micrometers(um)
mm to um: move decimal 3 places to the right
um to mm: move decimal 3 places to the left Y
YOU TRY:
The mosquito “sucks” blood using a proboscis. This mosquito has a proboscis that measures 1.34 mm. How large is the proboscis in micrometers (um)?
12/4/2018

9. 7. How to make a wet-mount slide …
1 – Get a clean slide and coverslip from your teacher.
2 – Place ONE drop of the creek water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess!
3 – Place the edge of the cover slip on one side of the water drop.
4 - Slowly lower the cover slip on top of the drop.
Cover Slip
Lower slowly
5 – Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives.
Do not use stage clips, try moving the slide around until you find something

10. FIRST READ THIS then follow directions on the worksheet for # 8
1 – Turn on the microscope and then rotate the nosepiece to click the red-banded objective into place.
2 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer.
3 – Once you have the image in view, rotate the nosepiece to view it under different powers. Draw what you see on your worksheet!
4 – When you are done, rinse both the cover slip and slide and return the materials. Turn off the microscope.

11. 8. Make a wet mount slide using the pond water provided
8. Make a wet mount slide using the pond water provided. Find an organism or image and draw what you see at three different powers of magnification. Label each drawing.
Note: When Drawing Specimens
Use pencil - you can erase and shade areas
All drawings should include clear and proper labels (and be large enough to view details). Drawings should be labeled with the specimen name and magnification.
Labels should be written on the outside of the circle. The circle indicates the viewing field as seen through the eyepiece, specimens should be drawn to scale - ie..if your specimen takes up the whole viewing field, make sure your drawing reflects that.

12. Internet Practice Activities these activities are on my webpage under “Scientific Method”
Quizlet: U&D Microscope Review for Quiz PowerPoint Parts of the Microscope Microscope Hangman