From DNA to Protein. Knowledge of Nucleic Acid Chemistry Is Essential to the Understanding of DNA Structure.

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Presentation transcript:

From DNA to Protein

Knowledge of Nucleic Acid Chemistry Is Essential to the Understanding of DNA Structure

Nucleotides are the building blocks of DNA. They consist of a nitrogenous base, a pentose sugar, and a phosphate group.

A nucleoside contains the nitrogenous base and the pentose sugar. A nucleotide is a nucleoside with a phosphate group added

The nitrogenous bases can be purines or pyrimidines. The purines are adenine (A) and guanine (G). The pyrimidines are cytosine (C), thymine (T), and uracil (U)

RNA contains ribose as its sugar; DNA contains deoxyribose The C-5' position is the location of the phosphate group on a nucleotide. DNA and RNA both contain A, C, and G, but only DNA contains T and only RNA contains U.

Nucleotides are linked by a phosphodiester bond between the phosphate group at the C-5' position and the OH group on the C-3' position

Nucleotides are linked by a phosphodiester bond between the phosphate group at the C-5' position and the OH group on the C-3' position

A polynucleotide chain

Antiparallel nature of the helix and the horizontal stacking of the bases.

Watson and Crick proposed DNA is a double helix in which the two strands are antiparallel and the bases are stacked on one another. The two strands are connected by A-T and G- C base pairing and there are 10 base pairs per helix turn

The DNA double helix as proposed by Watson and Crick

Polymerase Chain Reaction (PCR)