Microscope Parts & Function Light Microscope Electron Microscope
Ocular Lens (eyepiece) magnifies the image 10X Body Tube – distance between eyepiece & objectives. Arm – supports nosepiece
Objectives – further magnifies the image. 4X 10X 40X Revolving Nosepiece – holds the objectives, turns to change from one objective to another
Stage – supports slide Stage Clips – secure the slide in position for viewing
Coarse Adjustment Knob – moves the stage up and down in large increments to bring image into a rough focus. Fine Adjustment Knob – moves the stage slightly to bring image into sharp focus.
Diaphragm – controls the amount of light that is allowed to pass through the object being viewed Light Source – provides light for viewing the image Base – supports microscope
X axis stage adjustment – moves stage (and image) left & right. Y axis stage adjustment – moves stage (and image) up & down.
Microscope Use
Spider – 40X Human Hair - 200X Human Skin – 50X Flea – 40X
Spider – under electron microscope Deer hair (800X) electron microscope Skin – electron microscope Flea under electron microscope
Rules of Use
1. When moving your microscope, always carry it with both hands Grasp the arm with one hand and place the other hand under the base for support. 2. Turn the revolving nosepiece so that the lowest power objective lens (the shortest one) is "clicked" into position.
3. Place the microscope slide on the stage and fasten it with the stage clips. 4. Look at the microscope from the side and turn the coarse focus knob so that the stage moves all the way up as far as it will go without touching the slide!
5. Slowly turn the coarse adjustment (lowering the stage) until the image comes into focus. 6. Use the fine adjustment for fine focusing.
8. Adjust the light with the diaphragm for the clearest image. 7. Move the microscope slide around so that the image is in the center of the field of view 8. Adjust the light with the diaphragm for the clearest image. 9. Now, change to the next objective lens with only minimal use of the fine adjustment. 10. Do not use the coarse adjustment knob on any high power objective lens!
Do not touch the glass part of the lenses with your fingers. Keep the stage dry. When finished, move the low power lens back into position and remove the slide. Tie cord up when finished. Always keep your microscope covered when not in use.
Microscope Magnification
Image orientation
Depth of focus
Ocular lens (eyepiece) is 10X 4x objective lens 10x objective lens 40x objective lens To get total magnification you multiply the two together: (10X) (4X) = 40X magnification (10x) (10X) = 100X magnification (10X) (40X) = 400X magnification
Cork Cell 40X 100X 400X
Elodea Cell Leaf on slide 40X 100X 400X
Onion cell 10X 40X 400X
Cheek Cell 40X 100X 400X
Practice Question The lenses shown in the photo are known as what? A. projection lenses B. eyepiece lenses C. objective lenses D. power lenses
Practice Question All the lenses shown in the photo are part of a structure of the microscope known as the what? A. rotating lenspiece B. stage C. revolving nosepiece D. arm
Practice Question How is the total magnification of the microscope is obtained? A. multiplying the power of the objective lenses together. B. multiplying the power of the objective lens by the power of the eyepiece lens. C. dividing the power of the objective lens by the power of the eyepiece lens. D. dividing the power high power objective lens by the power of the low power objective lens.
Practice Question What is best used as an adjustment on a microscope to bring a specimen into focus at high power? A. Fine focus knob B. Objective lens C. Coarse focus knob D. Eyepiece