1. Microscopes

2. Types of Microscopes Compound light microscope
advantages
disadvantages
Transmission electron microscope
Scanning electron microscope

3. Compound Light Microscope
firegrass

4. Transmission electron microscope
Nerve of a pig
Bone marrow

5. Scanning electron microscope
Cat flea
Black widow spider claw
Leaf vein

6. Microscope Parts Ocular or eyepiece: contains a magnifying lens;
magnifies image 10X
Low Power Objective: Provides a magnification
of ___X and is the shortest of the objectives
Medium Objective: Provides a mag. of ___X.
High Objective: Provides a mag. of ___X and
is the longest of the objectives
Revolving Nosepiece: contains the
objectives and allows magnification to be adjusted
Slide: Holds the specimen in place
Stage: Supports the slide being observed
Cover Slip: goes over the specimen on the slide
keeping the specimen in place
Diaphragm: Regulates the amount of
light entering the body tube
Base: Supports the microscope
Illuminator/Light Source: Produces light or
reflects light up through the body tube
Coarse Adjustment: Moves the stage
up and down for focusing
Fine Adjustment: Moves the stage
slightly to sharpen focus
Stage Clips: Hold the slide in position
Arm: supports the body tube
Body Tube: Maintains a proper distance
between the eyepiece and the objective lenses

7. Microscope Terms Definition: clarity; how clear the specimen appears
Magnification: amount of enlargement of an image
Orientation: way in which something appears to be situated
Field of view: what is seen through the microscope
Depth of Field: refers to layers, 3-D effect that is viewable by changing fine adjustment under high power
Width of Field: distance across the diameter of your field of view, used to estimate the size of a specimen

8. Steps to Storing a Microscope
1.) Turn off light and allow to sit for 5 min to cool down.
2.) Turn the stage all the way down.
3.) Return objective to low.
4.) Remove slide. Rest the stage clips on the stage.
5.) Loosely wrap and secure the electrical cord.
6.) Turn the ocular over the body tube.
7.) Cover the microscope with the jacket.
8.) Carry the microscope with two hands.
9.) Place the microscope in the closet with the ocular pointing out.

9. Steps to Focusing a Microscope (Know in Order!!)
1.) Turn light on and leave it on for as long as you are using the microscope.
2.) Align the slide on the stage and secure with the stage clips.
3.) Turn the ocular towards you by rotating the base of the ocular, not the tube.
4.) Using the coarse adjustment, bring your specimen into view, and center it in your field.
5.) Adjust the diaphragm for the “best” lighting, not necessarily the brightest setting.
6.) Using your fine adjustment, sharpen your image (give the image definition.)
7.) Using the revolving nose piece, turn to your medium power objective.
8.) Using the coarse adjustment, bring your best image into view, center it, and turn the fine adjustment for definition.
9.) Turn the high power objective into place using the nosepiece. Using only the fine adjustment knob, fine tune your image.
10.) You may also want to add more light for a better image at this point.